RBP4 Protein (AA 19-201, C-Term)
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- 抗原 See all RBP4 蛋白
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 19-201, C-Term
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宿主
- 人
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资源
- CHO Cells
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determine by the LAL method.
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Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201) protein (His tag)
RBP4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201) protein (GST tag)RBP4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Retinol Binding Protein 4, Plasma (RBP4) (AA 103-201) protein (GST tag)RBP4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201) protein (His tag)RBP4 宿主: 人 宿主: HEK-293T Cells Recombinant SDS
Retinol Binding Protein 4, Plasma (RBP4) (Active) protein (His tag)RBP4 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Retinol Binding Protein 4, Plasma (RBP4) (Active) protein (His tag)RBP4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Retinol Binding Protein 4, Plasma (RBP4) (AA 19-194) protein (His tag)Crystallography grade RBP4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Retinol Binding Protein 4, Plasma (RBP4) (AA 19-197) protein (His tag)Crystallography grade RBP4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Retinol Binding Protein 4, Plasma (RBP4) (AA 19-199) protein (His tag)Crystallography grade RBP4 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of tryptophan fluorescence in RBP4. The ED50 of RBP4 to all-trans rentinoic acid is >0.5 μM.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. After dilution, the protein can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
- 浓度
- 200 μg/mL
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
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- 抗原
- RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
- 别名
- RBP4 (RBP4 产品)
- 别名
- RDCCAS Protein, RBP Protein, RNBP Protein, rbp4 Protein, MGC54038 Protein, srbp Protein, PRBP Protein, RBPA Protein, Rbp-4 Protein, fb23c12 Protein, rbp Protein, wu:fb23c12 Protein, wu:fb58d04 Protein, wu:fb72b04 Protein, zgc:86911 Protein, retinol binding protein 4 Protein, renin binding protein Protein, retinol binding protein 4 S homeolog Protein, retinol binding protein 4 A, plasma Protein, retinol binding protein 4, plasma Protein, retinol binding protein 4 L homeolog Protein, RBP4 Protein, RENBP Protein, rbp4.S Protein, rbp4 Protein, Rbp4 Protein, RBP4A Protein, rbp4.L Protein
- 背景
- RBP4 belongs to the lipocalin family, and it is the retinol (vitamin A alcohol) specific transport protein present in plasma. It has a β barrel structure with a well defined cavity, which accommodates retinol. The stability of the retinol-RBP (holo-RBP) complex is further enhanced when the complex is bound to transthyretin (TTR). RBP4 delivers retinol from the liver stores to the peripheral tissues. The interaction of RBP-retinol complex with TTR prevents its loss by filtration through the kidney glomeruli. Apo-RBP4 (without retinol) is reabsorbed in the proximal tubular cells. A deficiency of vitamin A blocks secretion of the binding protein post-translationally and results in defective delivery and supply to the epidermal cells. Defects in RBP4 cause retinol-binding protein deficiency and can cause night vision problems. RBP4 has been described as an adipokine and is found to be expressed in adipose tissue and correlated with obesity, insulin resistance, and type 2 diabetes. It has been postulated that RBP4 acts as the mechanistic link by which decreased adipocyte GLUT4 expression contributes to insulin resistance. Circulating RBP4 concentrations are increased in insulin-resistant mice. Moreover, transgenic overexpression of human RBP4 and injection of recombinant RBP4 decreased insulin sensitivity in normal mice, whereas genetic deletion of the RBP4 gene or normalization of RBP4 concentrations in obese mice improved insulin sensitivity. RBP4 concentrations are also increased in obese humans, and higher concentrations have been correlated with lower insulin sensitivity and other components of the metabolic syndrome.
- 分子量
- The 190 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The protein migrates as above 25 kDa in SDS-PAGE in DTT-reducing condition and above 22 kDa in non-reducing condition. The N-terminal amino acid is Gln.
- 途径
- Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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