Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201), (C-Term) (Active) 蛋白

ABIN2666743 产品详细信息, 供应商: Log in to see
蛋白名称
  • rbp4
  • MGC54038
  • srbp
  • 2310002G05Rik
  • 2610028L19Rik
  • HSRBP4
  • RBP4
  • RBP
  • RNBP
  • RDCCAS
  • Rbp-4
  • RBPA
  • PRBP
  • retinol binding protein 4, plasma
  • polymerase (RNA) II (DNA directed) polypeptide D
  • renin binding protein
  • rbp4
  • Polr2d
  • RENBP
  • RBP4
  • Rbp4
产品特性
AA 19-201, C-Term
24
6
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
宿主

34
8
5
5
3
2
2
2
1
1
1
1
1
资源
CHO Cells
22
10
9
7
6
5
2
2
蛋白类型
Recombinant
生物活性
Active
应用范围
Flow Cytometry (FACS)
选项
Supplier
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纯度 > 95 % , as determined by Coomassie stained SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平

Less than 0.1 EU per μg of protein as determine by the LAL method.

背景 RBP4 belongs to the lipocalin family, and it is the retinol (vitamin A alcohol) specific transport protein present in plasma. It has a β barrel structure with a well defined cavity, which accommodates retinol. The stability of the retinol-RBP (holo-RBP) complex is further enhanced when the complex is bound to transthyretin (TTR). RBP4 delivers retinol from the liver stores to the peripheral tissues. The interaction of RBP-retinol complex with TTR prevents its loss by filtration through the kidney glomeruli. Apo-RBP4 (without retinol) is reabsorbed in the proximal tubular cells. A deficiency of vitamin A blocks secretion of the binding protein post-translationally and results in defective delivery and supply to the epidermal cells. Defects in RBP4 cause retinol-binding protein deficiency and can cause night vision problems. RBP4 has been described as an adipokine and is found to be expressed in adipose tissue and correlated with obesity, insulin resistance, and type 2 diabetes. It has been postulated that RBP4 acts as the mechanistic link by which decreased adipocyte GLUT4 expression contributes to insulin resistance. Circulating RBP4 concentrations are increased in insulin-resistant mice. Moreover, transgenic overexpression of human RBP4 and injection of recombinant RBP4 decreased insulin sensitivity in normal mice, whereas genetic deletion of the RBP4 gene or normalization of RBP4 concentrations in obese mice improved insulin sensitivity. RBP4 concentrations are also increased in obese humans, and higher concentrations have been correlated with lower insulin sensitivity and other components of the metabolic syndrome.
分子量 The 190 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The protein migrates as above 25 kDa in SDS-PAGE in DTT-reducing condition and above 22 kDa in non-reducing condition. The N-terminal amino acid is Gln.
研究领域 Cardiovascular, Atherosclerosis, Metabolism, Hormones, Cytokines
途径 Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
应用备注 Optimal working dilution should be determined by the investigator.
说明

Biological activity: Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of tryptophan fluorescence in RBP4. The ED50 of RBP4 to all-trans rentinoic acid is >0.5 μM.

限制 仅限研究用
状态 Liquid
溶解方式 For maximum results, quick spin vial prior to opening. After dilution, the protein can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.
浓度 200 μg/mL
缓冲液 0.22 μm filtered protein solution is in PBS.
注意事项 Avoid repeated freeze/thaw cycles.
储存条件 -20 °C
储存方法 Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.
厂商提供的图像
ELISA image for Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201), (C-Term) (Active) protein (ABIN2666743) Retinol Binding Protein 4, Plasma (RBP4) (AA 19-201), (C-Term) (Active) protein
背景 Kotnik, Keuper, Wabitsch, Fischer-Posovszky: "Interleukin-1β downregulates RBP4 secretion in human adipocytes." in: PLoS ONE, Vol. 8, Issue 2, pp. e57796, 2013 (PubMed).

Tepper, Kim, Shete, Shabrova, Quadro: "Serum retinol-binding protein 4 (RBP4) and retinol in a cohort of borderline obese women with and without gestational diabetes." in: Clinical biochemistry, Vol. 43, Issue 3, pp. 320-3, 2010 (PubMed).

Frey, Nagl, Henze, Raila, Schlosser, Berg, Tepel, Zidek, Weickert, Pfeiffer, Schweigert: "Isoforms of retinol binding protein 4 (RBP4) are increased in chronic diseases of the kidney but not of the liver." in: Lipids in health and disease, Vol. 7, pp. 29, 2008 (PubMed).

Yang, Graham, Mody, Preitner, Peroni, Zabolotny, Kotani, Quadro, Kahn: "Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes." in: Nature, Vol. 436, Issue 7049, pp. 356-62, 2005 (PubMed).

Zanotti, Berni: "Plasma retinol-binding protein: structure and interactions with retinol, retinoids, and transthyretin." in: Vitamins and hormones, Vol. 69, pp. 271-95, 2004 (PubMed).

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