Prolactin Protein (PRL) (AA 29-227, N-Term)
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- 抗原 See all Prolactin (PRL) 蛋白
- Prolactin (PRL)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 29-227, N-Term
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Flow Cytometry (FACS)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.1 EU per μg of protein as determined by the LAL method.
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- Discover our top product PRL 蛋白
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Prolactin (PRL) (Active) protein
PRL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Prolactin (PRL) (Active) protein (His tag)PRL 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing SDS-PAGE. Active
Prolactin (PRL) protein (His tag)PRL 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Prolactin (PRL) (AA 29-227) protein (GST tag)PRL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Prolactin (PRL) protein (His tag)PRL 宿主: 绵羊 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Prolactin (PRL) (AA 29-227) protein (His tag)Crystallography grade PRL 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prolactin (PRL) (AA 30-226) protein (His tag)Crystallography grade PRL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prolactin (PRL) (AA 29-227) protein (Fc Tag)PRL 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Prolactin (PRL) (AA 29-227) protein (Fc Tag,AVI tag,Biotin)PRL 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Human Prolactin induces proliferation of MCF7 breast cancer cells in a dose dependent manner. The ED50 is 60 - 350 ng/mL.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- Prolactin (PRL)
- 别名
- Prolactin (PRL 产品)
- 别名
- PRL Protein, prolactin Protein, PRLB Protein, PRLSD1 Protein, Prl1a1 Protein, Prol Protein, RATPRLSD1 Protein, RNPROL Protein, AV290867 Protein, prl Protein, prolactin Protein, prolactin, gene 2 L homeolog Protein, PROLACTIN protein Protein, prolactin-like Protein, PRL Protein, prl.2.L Protein, Prl Protein, LOC100136792 Protein, LOC100136580 Protein, PROLACTIN Protein, LOC101843376 Protein
- 背景
- Prolactin, also known as luteotropin, is a hormone that is secreted by the pituitary gland and is best known for its role in enabling female mammals to produce milk. However, prolactin has also been shown to play essential roles in the regulation of immune functions, pancreatic development, angiogenesis, reproduction, osmoregulation, and metabolism. Besides the pituitary origin, prolactin is found in other tissues and cells such as adipocytes, immune cells, skin, prostate, brain, and decidua. This hormone is produced in increasing amounts during pregnancy and suckling. It acts on the mammary gland by initiating and maintaining lactation in the postpartum period. Epidemiological studies have demonstrated that circulating high levels of prolactin increase the risk of breast cancer. Prolactin may promote mammary tumor initiation via the JAK2/STAT5 signaling pathway. Human prolactin has a 60 % amino acid sequence homology with its murine counterpart. In a knockout animal study, it was shown that female mice lacking the expression of prolactin were infertile, but does not prevent them from manifesting spontaneous maternal behaviors. In addition, these animals show no sign of alterations in myelopoiesis and lymphopoiesis, suggesting that prolactin may not play an indispensable role in lymphocyte development and myeloid differentiation.
- 分子量
- The 200 amino acid recombinant protein has a predicted molecular mass of approximately 23 kDa. The protein migrates approximately at 25 kDa in DTT-reducing conditions by SDS-PAGE. The predicted N-terminal amino acid is Met.
- 途径
- JAK/STAT Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Protein targeting to Nucleus
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