COL18A1 Protein (AA 1154-1335, N-Term)
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- 抗原 See all COL18A1 蛋白
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1154-1335, N-Term
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
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Collagen, Type XVIII, alpha 1 (COL18A1) protein (His tag)
COL18A1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1572-1754) protein (His tag)Crystallography grade COL18A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1591-1774) protein (His tag)Crystallography grade COL18A1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Collagen, Type XVIII, alpha 1 (COL18A1) protein (KLH)COL18A1 宿主: 人 宿主: 合成 > 95 % SDS, WB, ELISA, IP
Collagen, Type XVIII, alpha 1 (COL18A1) (Transcript Variant 2) (Active) proteinCOL18A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1578-1754) protein (GST tag)COL18A1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1578-1754) protein (His tag)COL18A1 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: ED50 = 0.5 - 3 μg/ml, as determined by inhibition of HUVEC cell proliferation induced by human FGF basic.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- 缓冲液
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- 抗原
- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
- 别名
- Endostatin (COL18A1 产品)
- 别名
- KNO Protein, KNO1 Protein, KS Protein, cb371 Protein, cb529 Protein, fc96d06 Protein, sb:cb529 Protein, wu:fb47h10 Protein, wu:fb77g12 Protein, wu:fc50f04 Protein, wu:fc96d06 Protein, wu:fk02h12 Protein, kno Protein, kno1 Protein, collagen type XVIII alpha 1 chain Protein, collagen type XVIII alpha 1 chain a Protein, collagen, type XVIII, alpha 1 Protein, collagen type XVIII alpha 1 Protein, COL18A1 Protein, col18a1a Protein, Col18a1 Protein, col18a1 Protein
- 物质类
- Chemical
- 背景
- Endostatin is a C-terminal proteolytic fragment of collagen XVIII α1 (Col 18) that is localized in the vascular basement membrane of almost all epithelia and endothelia. Endostatin is produced from catalytic cleavage from MMPs, cathepsin L, and S. It is detected in circulation at a physiologic level of 20 to 50 ng/mL in serum. Crystal X-ray analysis of endostatin has shown a compact globular structure, with one face rich in arginine residues that acts as a heparin-binding motif. Endostatin interacts with multiple molecules including heparin, heparan sulfate, chondroitin sulfate, dermatan sulfate, thrombospondin-1, SPARC, collagens (I, IV, and VI), the amyloid peptide Aβ (1-42), transglutaminase-2, and among others. Endostatins derived from collagens XV and XVIII differ in structure and binding properties, tissue distribution, and anti-angiogenic activity. Endostatin is an endogenous angiostatic factor that induces apoptosis in endothelial cells and inhibits endothelial cell migration in vitro. Col 18-null mice showed faster tumor growth when implanted with tumor cells that do not produce Col 18. Overexpression of circulating endostatin in transgenic mice resulted in reduced tumor growth. Experimental model of glomerulonephritis in Col 18-null mice showed that the absence of this molecule exacerbates immune-mediated glomerulonephritis.
- 分子量
- The 192 amino acid recombinant protein has a predicted molecular mass of approximately 21.3 kDa. The DTT-reduced and non-reduced protein migrate at approximately 23 and 20 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.
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