GZMB Protein (AA 19-249)
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- 抗原 See all GZMB 蛋白
- GZMB (Granzyme B (GZMB))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 19-249
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宿主
- 人
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资源
- HEK-293 Cells
- 应用范围
- Intracellular Flow Cytometry (ICFC)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
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Less than 1.0 EU per μg of protein as determine by the LAL method.
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Granzyme B (GZMB) (AA 21-247) protein (GST tag)
GZMB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Granzyme B (GZMB) (AA 148-247) protein (GST tag)GZMB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Granzyme B (GZMB) protein (His tag)GZMB 宿主: 小鼠 宿主: 人细胞 Recombinant > 85 % as determined by SDS-PAGE
Granzyme B (GZMB) (AA 1-247) protein (GST tag)GZMB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Granzyme B (GZMB) (AA 21-247) protein (His tag)Crystallography grade GZMB 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Granzyme B (GZMB) (AA 21-247) protein (His tag)Crystallography grade GZMB 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Human Granzyme B activated by mouse Cathepsin C is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/μg.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- For maximum results, quick spin vial prior to opening.
- 缓冲液
- 0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, pH 7.5.
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -70°C for six months.
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- 抗原
- GZMB (Granzyme B (GZMB))
- 别名
- Granzyme B (GZMB 产品)
- 别名
- CCPI Protein, CGL-1 Protein, CGL1 Protein, CSP-B Protein, CSPB Protein, CTLA1 Protein, CTSGL1 Protein, HLP Protein, SECT Protein, granzyme B Protein, GZMB Protein
- 背景
- Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumorous cells. This is evident by the fact that CTLs from mice with inhibited granzyme B production exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T lymphocytes induce substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells, including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardia infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.
- 分子量
- This 244 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kDa. The protein migrates at about 37 kDa in DTT-reducing conditions and about 37 kDa in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid i
- 途径
- Apoptosis, Caspase Cascade in Apoptosis
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