NOG Protein (AA 28-232)
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- 抗原 See all NOG 蛋白
- NOG (Noggin (NOG))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 28-232
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宿主
- 人
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资源
- HEK-293 Cells
- 应用范围
- Western Blotting (WB)
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 内毒素水平
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Less than 0.1 ng per μg of protein.
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Noggin (NOG) (AA 20-232) (Active) protein
NOG 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95% as determined by Tris-Bis PAGE
Noggin (NOG) (AA 28-232) (Active) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) protein (His tag)NOG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (His tag)NOG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Noggin (NOG) (AA 28-232) protein (His tag)Crystallography grade NOG 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Noggin (NOG) (AA 28-232) protein (His tag)Crystallography grade NOG 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Biological activity: Recombinant human Noggin is able to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC5 chondrogenic cells. The ED50 is 2.0 - 3.0 ng/ml, corresponding to a specific activity of 3.3 x 105 - 5.0 x 105 units/mg.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- 缓冲液
- Lyophilized
- 注意事项
- Avoid repeated freeze/thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Unopened vial can be stored at -20°C or -70°C.
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- 抗原
- NOG (Noggin (NOG))
- 别名
- Noggin (NOG 产品)
- 别名
- SYM1 Protein, SYNS1 Protein, nog-A Protein, nog1 Protein, noggin-1 Protein, noggin Protein, noggin Protein, noggin L homeolog Protein, noggin protein Protein, NOG Protein, Nog Protein, nog.L Protein, noggin Protein
- 背景
- Noggin (NOG) is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. NOG also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of NOG to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. NOG also appears to maintain adult stem cell populations in vivo. For example, it maintains neural stem cells within the hippocampus. Targeted deletion of NOG in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOG in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
- 分子量
- Recombinant human Noggin is a 46 kDa disulfide-linked homodimer consisting of two 205 amino acid polypeptide chains. Monomeric glycosylated Noggin migrates at an apparent molecular weight of approximately 28 - 33 kDa by SDS PAGE analysis under reducing co
- 途径
- Stem Cell Maintenance, Tube Formation
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