Product: Full length recombinant mature FGF-1 (15.8 kDa, 140 amino acids) expressed in E. coli. Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit (Cell Titer 96, Promega) with Balb/c3T3 cells was < 10 pg/mL, corresponding to a specific activity of < 1 x 108 units/mg.
FGF1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % by SDS-PAGE.
Active
应用备注
1. Use as a supplement in serum-free or reduced serum media for culture of mammalian cells 2. Studies of the human FGF-1 receptor, transmembrane signaling and protein phosphorylation 3. Western blotting control for anti-EGF-1 antibodies 4. Widely used in cosmetics,such as whitening,anti-wrinkle,anti-aging,etc.
限制
仅限研究用
状态
Liquid
浓度
2 mg/mL
缓冲液
PBS (10 mM Na-phosphate, 150 mM NaCl) pH 7.2, 50 % glycerol
储存条件
-20 °C/-80 °C
储存方法
-20 C (For long term storage: -70 C)
Zakrzewska, Marcinkowska, Wiedlocha: "FGF-1: from biology through engineering to potential medical applications." in: Critical reviews in clinical laboratory sciences, Vol. 45, Issue 1, pp. 91-135, (2008) (PubMed).
FGF-1 (acidic FGF) is a member of the fibroblast growth factor (FGF) family, which binds heparin. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.