Diphtheria Toxin (Active) 蛋白

ABIN2452169 产品详细信息, 供应商: Log in to see
蛋白名称
宿主
Corynebacterium diphtheriae
资源
Corynebacterium diphtheriae
蛋白类型
Native
生物活性
Active
应用范围
Blocking Antibody (Inhibition), Functional Studies (Func), Negative Control (NC), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
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纯度 > 95 % purity by SDS-PAGE (see below, SDS-PAGE without mercaptoethanol)
背景 This Diphtheria toxin was highly purified from the growth media of Corynebacterium diphtheriae strain PW8 as mostly unnicked form. Diphtheria toxin is a single polypeptide chain of 535 amino acids (58 kD) and nicked by cellular protease like furin to give fragments A (N-terminal, 21 kDa) and B (C-terminal, 37 kDa) which are linked by disulfide bridges. Binding to the cell surface of frgment B allows fragment A to penetrate the host cell. Fragment A catalyzes the ADP-ribosylation of eucaryotic elongation factor-2 (eEF2) by using NAD as a substrate, thus inactivating eEF2 and inhibiting protein synthesis.
UniProt Q5PY51
研究领域 Toxins, Toxicology
应用备注 1) Inhibition of protein synthesis in eucaryotic cells
2) Negative selection agent for ES cells in construction of transgenic mouse
3) Putative drug for treatment of malignant tumors such as leukemia
4) Antigen for Western blotting and ELISA
5) As a marker of Diphtheria toxin in SDS-PAGE
说明

Activity test: Addition of 20 ~30 pg/mL of Diphtheria toxin in growth medium caused 50 % lethality after 43 h in Vero cells. Nicking of Diphtheria toxin by trypsin by trypsin and reduction by dithiothreitol further increase the activity

限制 仅限研究用
状态 Liquid
浓度 5 mg/mL
缓冲液 20 mM Tris-HCl ( pH 7.2), 150 mM NaCl
储存条件 -80 °C
厂商提供的图像
SDS-PAGE (SDS) image for Diphtheria Toxin (Active) protein (ABIN2452169) Diphtheria Toxin (Active) protein
有引用在: Yagi, Nada, Watanabe, Tamemoto, Kohmura, Ikawa, Aizawa: "A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene." in: Analytical biochemistry, Vol. 214, Issue 1, pp. 77-86, 1994 (PubMed).

Pappenheimer: "Diphtheria toxin." in: Annual review of biochemistry, Vol. 46, pp. 69-94, 1977 (PubMed).

Uchida, Pappenheimer, Harper: "Diphtheria toxin and related proteins. 3. Reconstitution of hybrid "diphtheria toxin" from nontoxic mutant proteins." in: The Journal of biological chemistry, Vol. 248, Issue 11, pp. 3851-4, 1973 (PubMed).

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