Carboxypeptidase E (CPE) (AA 26-453) (Active) protein (His tag)

ABIN2180678 产品详细信息, 供应商: Log in to see
蛋白名称
  • CPE
  • CPH
  • Cph-1
  • Cph1
  • R74677
  • fat
  • CARBE
  • Cph
  • si:ch211-198g9.2
  • zgc:85981
  • carboxypeptidase E
  • CPE
  • cpe
  • Cpe
产品特性
AA 26-453
5
2
2
1
1
1
1
1
1
1
宿主

17
5
1
1
1
1
1
资源
HEK-293 Cells
6
5
5
4
2
2
1
1
1
蛋白类型
Recombinant
生物活性
Active
标记
His tag
选项
Supplier
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产品特性 This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 49 kDa. The protein migrates as 53 kDa under reducing (R) condition (SDS-PAGE).
纯度 >95 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to cleave a peptide substrate, benzoylAROH. The product, Arg, is reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is > 12000 pmoles/min/μg.
背景 Carboxypeptidase E (CPE) is also known as Carboxypeptidase H or enkephalin convertase. Carboxypeptidase E cleaves C-terminal amino acid residues and is involved in neuropeptide processing. It is a peripheral membrane protein. CPE specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Carboxypeptidase E appears to have several functions. The active form of carboxypeptidase E was shown to be in secretory vesicles, where it acts as an exopeptidase to activate neuropeptides, It does that by cleaving off basic C-terminal amino acids, producing the active form of the peptide. Products of carboxypeptidase E include insulin, enkephalin, and most other neuroendocrine peptides.It has also been proposed that membrane-associated carboxypeptidase E acts as a sorting signal for regulated secretory proteins in the trans-Golgi network of the pituitary and in secretory granules, regulated secretory proteins are mostly hormones and neuropeptides. Mice with mutant carboxypeptidase E Cpe, display endocrine disorders like obesity and infertility.
分子量 49 kDa
NCBI登录号 NP_001864
UniProt P16870
研究领域 Enzymes, Metabolism
途径 Peptide Hormone Metabolism, Synaptic Membrane
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 PBS, pH 7.4
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for Carboxypeptidase E (CPE) (AA 26-453) (Active) protein (His tag) (ABIN2180678) Human Carboxypeptidase E, His Tag on SDS-PAGE under reducing (R) condition. The gel w...
背景 Jeffrey, Alejandro, Luciani, Kalynyak, Hu, Li, Lin, Townsend, Polonsky, Johnson: "Carboxypeptidase E mediates palmitate-induced beta-cell ER stress and apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 24, pp. 8452-7, 2008 (PubMed).

Tai, Foster, Rosales, Feng, Hasilo, Martinez, Ramadan, Snir, Melling, Dhanvantari, Rutt, White: "Imaging islets labeled with magnetic nanoparticles at 1.5 Tesla." in: Diabetes, Vol. 55, Issue 11, pp. 2931-8, 2006 (PubMed).

Dhanvantari, Loh: "Lipid raft association of carboxypeptidase E is necessary for its function as a regulated secretory pathway sorting receptor." in: The Journal of biological chemistry, Vol. 275, Issue 38, pp. 29887-93, 2000 (PubMed).

Manser, Fernandez, Loo, Goh, Monfries, Hall, Lim: "Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro." in: The Biochemical journal, Vol. 267, Issue 2, pp. 517-25, 1990 (PubMed).

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