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Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-344) (Active) protein (Fc Tag)

ABIN2180572 产品详细信息, 供应商: Log in to see
蛋白名称
  • AGER
  • RAGE
  • advanced glycosylation end product-specific receptor
  • AGER
  • Ager
  • LOC100343142
产品特性
AA 23-344
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
宿主

31
5
2
1
资源
HEK-293 Cells
10
4
4
4
4
3
2
2
1
1
蛋白类型
Recombinant
生物活性
Active
标记
Fc Tag
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产品特性 This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 60.8 kDa. The protein migrates as 80 - 90 kDa and 35 kDa Fc fragment under reducing (R) condition (SDS-PAGE) due to glycosylation and auto-cleavage.
纯度 >60 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized human S100A12 at 2 μg/mL ( 100 μL/well ) can bind recombinant human AGER with a linear range of 0.032 - 20μg/mL

SDS-PAGE: due to glycosylation and auto-cleavage.
背景 Advanced glycosylation end product-specific receptor (AGER) is also known as RAGE, and is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. AGER is highly expressed in endothelial cells. AGER mediates interactions of advanced glycosylation end products (AGE), and also accumulates in vascular tissue in aging and at an accelerated rate in diabetes, as well as acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGER / RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.
分子量 60.8 kDa
NCBI登录号 NP_001127
UniProt Q15109
研究领域 Neurology, Alzheimer's Disease
途径 Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration
限制 仅限研究用
状态 Lyophilized
溶解方式 Please see Certificate of Analysis for specific instructions. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 50 mM Tris, 100 mM Glycine, pH 7.5
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-344) (Active) protein (Fc Tag) (ABIN2180572) Human RAGE, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained over...
背景 Xue, Rai, Singer, Chabierski, Xie, Reverdatto, Burz, Schmidt, Hoffmann, Shekhtman: "Advanced glycation end product recognition by the receptor for AGEs." in: Structure (London, England : 1993), Vol. 19, Issue 5, pp. 722-32, 2011 (PubMed).

Jin, Chen, Luo, Ding, Liu: "S100A14 stimulates cell proliferation and induces cell apoptosis at different concentrations via receptor for advanced glycation end products (RAGE)." in: PLoS ONE, Vol. 6, Issue 4, pp. e19375, 2011 (PubMed).

Fang, Lue, Yan, Xu, Luddy, Chen, Walker, Stern, Yan, Schmidt, Chen, Yan: "RAGE-dependent signaling in microglia contributes to neuroinflammation, Abeta accumulation, and impaired learning/memory in a mouse model of Alzheimer's disease." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 4, pp. 1043-55, 2010 (PubMed).