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Adiponectin (ADIPOQ ) (AA 19-244) (Active) protein (His tag)

ABIN2180564 产品详细信息, 供应商: Log in to see
蛋白名称
产品特性
AA 19-244
14
14
9
7
6
4
3
3
3
3
2
1
1
1
1
1
1
1
宿主

75
33
10
9
4
2
1
1
资源
HEK-293 Cells
73
11
8
5
4
4
4
2
2
2
2
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
标记
His tag
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产品特性 This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.4 kDa. The protein migrates as 28-33 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
纯度 >92 % as determined by SDS-PAGE.
过滤 0.22 μm filtered
内毒素水平 Less than 1.0 EU per μg by the LAL method.
ProductDetails: Biological Activity Comment Biological Activity: Measured by its ability to induce TIMP-1 secretion by mouse macrophages. The ED50 for this effect is typically 2 - 10 μg/mL.
背景 Adiponectin is also referred to as GBP-28, APM1, ADIPOQ, ACDC and Acrp30, is a secreted protein which contains one C1q domain (commonly called the globular domain) and onecollagen-like domain. Adiponectin / ADIPOQ is synthesized exclusively by adipocytes and secreted into plasma. ADIPOQ is important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Adiponectin antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Acrp30 Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway and may play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
分子量 25.4 kDa
NCBI登录号 NP_001171271
UniProt Q15848
研究领域 Cancer, Atherosclerosis, Metabolism, Hormones
限制 仅限研究用
状态 Lyophilized
溶解方式 For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
缓冲液 PBS, pH 7.4
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).
厂商提供的图像
SDS-PAGE (SDS) image for Adiponectin  (ADIPOQ ) (AA 19-244) (Active) protein (His tag) (ABIN2180564) Human Adiponectin, His Tag on SDS-PAGE under reducing (R) condition. The gel was stai...
背景 Richards, Stephens, Charlton, Jones, Macdonald, Prins, Whitehead: "Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: evidence for regulation of multimerization by alterations in posttranslational modifications." in: Molecular endocrinology (Baltimore, Md.), Vol. 20, Issue 7, pp. 1673-87, 2006 (PubMed).

Yamauchi, Kamon, Waki, Terauchi, Kubota, Hara, Mori, Ide, Murakami, Tsuboyama-Kasaoka, Ezaki, Akanuma, Gavrilova, Vinson, Reitman, Kagechika, Shudo, Yoda, Nakano, Tobe, Nagai, Kimura, Tomita, Froguel et al.: "The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. ..." in: Nature medicine, Vol. 7, Issue 8, pp. 941-6, 2001 (PubMed).

Nakano, Tobe, Choi-Miura, Mazda, Tomita: "Isolation and characterization of GBP28, a novel gelatin-binding protein purified from human plasma." in: Journal of biochemistry, Vol. 120, Issue 4, pp. 803-12, 1997 (PubMed).