Oncostatin M (OSM) (AA 26-221) (Active) 蛋白

ABIN2039658 产品详细信息, 供应商: Log in to see
蛋白名称
  • OSM
  • OncoM
  • oncostatin M
  • oncostatin M-like
  • oncostatin-M-like
  • OSM
  • Osm
  • LOC100342491
  • LOC100152038
产品特性
AA 26-221
6
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
宿主

56
9
5
1
资源
HEK293
50
6
3
2
1
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
应用范围
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
选项
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序列 AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRR
产品特性 rhOSM tested in Stat3 activation assay using glioblastoma U87 cell line.
ED50: 2.5-3 ng/mL
纯化方法 Produced and purified from Serum Free/ Animal Free media
纯度 >99 % , (according to SDS-PAGE stained with SimplyBlue SafeStain, (Invitrogen))
过滤 0.2 μm filtered
内毒素水平 Less than 0.1 ng/μg (1 IEU/μg), as measured by LAL method
背景 Oncostatin M is a member of the gp130 (IL-6/LIF) family of cytokines. It is naturally processed from 252 amino acid pre-pro OSM polypeptide. Propeptide processing is believed not to be important for OSM receptor binding activity, but may be important for growth-inhibitory activity. Oncostatin M variable cytokine activity depends on the target cell. Oncostatin M was initially identified as a cytokine capable of inhibiting the in vitro growth of melanoma cells and solid tumors (1). It has been subsequently shown to promote proliferation of ovarian cancer cells (2) prostate cancer 22Rv1 cells (3) and AIDS-KS cells (4), up-regulate the ER chaperone Grp78/BiP in the liver cells (5), inhibit proliferation of breast cancer cell lines (6,7) and induce the activation of signaling pathways Jak2, Stat3, and Erk1/2 (8). OSM is normally expressed in macrophages, monocytes, T cells, and dendritic cells (8-10).
分子量 22.151 kDa
研究领域 Immunology, Innate Immunity, Cytokines, Signaling, Receptors
途径 JAK/STAT Signaling, Negative Regulation of Hormone Secretion
应用备注 22 kDa, under reducing conditions.
说明

outstanding bioactivity, superior lot-to-lot reproducibilty in bioactivity assays, sertificate of analysis for every lot.

限制 仅限研究用
状态 Lyophilized
溶解方式 Spin before opening. For optimal recovery - reconstitute in sterile water at ~0.1 mg/mL at room temperature, after adding water, re-cap the vial and tap gently, ensure to cover all the surfaces inside the vial. Do not mix by vortexing or by extensive pipetting. Let the temperature with gentle agitation for at least 10-15 minutes before aliquoting or using.vial to sit at room
缓冲液 Dried from 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
注意事项 Avoid repeated freeze-thaw cycles.
Not for use in humans.
储存条件 -20 °C/-80 °C
储存方法 To avoid loss of the protein, store the reconstituted protein in aliquots (no smaller than 10 μL) in polypropylene or siliconized tubes. Store dried and reconstituted protein at -20 °C and/or -80 °C.
厂商提供的图像
 image for Oncostatin M (OSM) (AA 26-221) (Active) protein (ABIN2039658) Oncostatin M (OSM) (AA 26-221) (Active) protein
有引用在: Godoy-Tundidor, Cavarretta, Fuchs, Fiechtl, Steiner, Friedbichler, Bartsch, Hobisch, Culig: "Interleukin-6 and oncostatin M stimulation of proliferation of prostate cancer 22Rv1 cells through the signaling pathways of p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase." in: The Prostate, Vol. 64, Issue 2, pp. 209-16, 2005 (PubMed).

Yin, Chain: "Suppression of lymphokine production in anti-minor histocompatibility antigen responses." in: Cytokine, Vol. 3, Issue 1, pp. 5-11, 1991 (PubMed).

Malik, Kallestad, Gunderson, Austin, Neubauer, Ochs, Marquardt, Zarling, Shoyab, Wei: "Molecular cloning, sequence analysis, and functional expression of a novel growth regulator, oncostatin M." in: Molecular and cellular biology, Vol. 9, Issue 7, pp. 2847-53, 1989 (PubMed).

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