EPH Receptor A2 (EPHA2) (Extracellular Domain), (AA 1-535) (Active) protein (His tag)

ABIN2008013 产品详细信息, 供应商: Log in to see
蛋白名称
  • EPHA2
  • epha2
  • MGC89505
  • ARCC2
  • CTPA
  • CTPP1
  • CTRCT6
  • ECK
  • AW545284
  • Eck
  • Myk2
  • Sek-2
  • Sek2
  • EPH receptor A2
  • EphA2
  • epoxide hydrolase
  • Eph receptor A2
  • EPHA2
  • epha2
  • ephA2
  • Epha2
产品特性
Extracellular Domain, AA 1-535
3
2
1
1
1
1
1
1
1
1
1
1
宿主
小鼠
13
3
资源
人细胞
6
2
2
2
2
1
1
蛋白类型
Recombinant
生物活性
Active
标记
This EPH Receptor A2 protein is labelled with His tag.
应用范围
Functional Studies (Func), SDS-PAGE (SDS)
选项
Supplier
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产品特性 The secreted recombinant mouse EPHA2 consists of 523 amino acids and has a predicted molecular mass of 58 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEPHA2 is approximately 65 kDa due to glycosylation.

Protein Structure: A DNA sequence encoding the mouse EPHA2 (NP_034269.2) extracellular domain (Met 1-Asn 535) was expressed, fused with a polyhistidine tag at the C-terminus.
Predicted N-Term: Gln 24
纯度 > 98 % as determined by SDS-PAGE
内毒素水平 < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its binding ability in a functional ELISA.
1. Immobilized mouse EphA2 at 2μg/ml (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/ml.
2. Immobilized mouse EphA2 at 2 μg/ml (100 μl/well) can bind human EphrinA1 with a linear range of 0.8-20 ng/ml.
背景 Synonyms: AW545284,Eck,Myk2,Sek-2,Sek2
分子量 58 kDa, 65 kDa
NCBI登录号 NP_034269
研究领域 Signaling
途径 RTK signaling
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
缓冲液 Lyophilized from sterile 20 mM Tris, 150 mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
注意事项 Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
储存条件 -20 °C,-80 °C
储存方法 Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
有效期 12 months
厂商提供的图像
 image for EPH Receptor A2 (EPHA2) (Extracellular Domain), (AA 1-535) (Active) protein (His tag) (ABIN2008013) EPH Receptor A2 (EPHA2) (Extracellular Domain), (AA 1-535) (Active) protein (His tag)
背景 Zhang, Njauw, Park, Naruse, Asano, Tsao: "EphA2 is an essential mediator of UV radiation-induced apoptosis." in: Cancer research, Vol. 68, Issue 6, pp. 1691-6, 2008 (PubMed).

Rikova, Guo, Zeng, Possemato, Yu, Haack, Nardone, Lee, Reeves, Li, Hu, Tan, Stokes, Sullivan, Mitchell, Wetzel, Macneill, Ren, Yuan, Bakalarski, Villen, Kornhauser, Smith, Li, Zhou, Gygi, Gu et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. ..." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).

Gerhard, Wagner, Feingold, Shenmen, Grouse, Schuler, Klein, Old, Rasooly, Good, Guyer, Peck, Derge, Lipman, Collins, Jang, Sherry, Feolo, Misquitta, Lee, Rotmistrovsky, Greenhut, Schaefer, Buetow et al.: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). ..." in: Genome research, Vol. 14, Issue 10B, pp. 2121-7, 2004 (PubMed).

Murai, Pasquale: "'Eph'ective signaling: forward, reverse and crosstalk." in: Journal of cell science, Vol. 116, Issue Pt 14, pp. 2823-32, 2003 (PubMed).

Nowakowski, Cronin, McRee, Knuth, Nelson, Pavletich, Rogers, Sang, Scheibe, Swanson, Thompson: "Structures of the cancer-related Aurora-A, FAK, and EphA2 protein kinases from nanovolume crystallography." in: Structure (London, England : 1993), Vol. 10, Issue 12, pp. 1659-67, 2002 (PubMed).