TIMP Metallopeptidase Inhibitor 1 (TIMP1) (AA 1-207) (Active) protein (His tag)

ABIN2003615 产品详细信息, 供应商: Log in to see
蛋白名称
  • TIMP-1
  • TIMP1
  • DKFZp468A0912
  • CLGI
  • EPA
  • EPO
  • HCI
  • TIMP
  • Timp
  • Clgi
  • TIMP metallopeptidase inhibitor 1
  • tissue inhibitor of metalloproteinase 1
  • TIMP1
  • Timp1
  • LOC100009047
产品特性
AA 1-207
20
3
3
2
1
1
1
1
1
1
1
1
1
1
1
宿主

32
6
3
2
2
2
2
1
1
1
1
资源
人细胞
22
8
6
5
4
4
4
1
1
1
1
蛋白类型
Recombinant
生物活性
Active
标记
This TIMP1 protein is labelled with His tag.
应用范围
Functional Studies (Func), SDS-PAGE (SDS)
选项
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产品特性 The secreted recombinant human TIMP1 comprises 195 amino acids with a predicted molecular mass of 22 kDa. As a result of glycosylation, rhTIMP1 migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human TIMP1 (NP_003245.1) (Met 1-Ala 207) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Cys 24
纯度 > 97 % as determined by SDS-PAGE
内毒素水平 < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 . The IC50 value is < 6 nM.
背景 Synonyms: CLGI,EPA,EPO,HCI,TIMP,TIMP-1
分子量 22 kDa, 30 kDa
NCBI登录号 NP_003245
研究领域 Proteolysis / Ubiquitin, Angiogenesis, Extracellular Matrix, Matrix Metalloproteinases, Cancer, Inflammation
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
状态 Lyophilized
缓冲液 Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
注意事项 Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
储存条件 -20 °C,-80 °C
储存方法 Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
有效期 12 months
厂商提供的图像
 image for TIMP Metallopeptidase Inhibitor 1 (TIMP1) (AA 1-207) (Active) protein (His tag) (ABIN2003615) TIMP Metallopeptidase Inhibitor 1 (TIMP1) (AA 1-207) (Active) protein (His tag)
背景 Aljada, Ramnath, Donohue, Harvey, Brooks, Wiseman, Khoury, Loewen, Slocum, Anderson, Bepler, Tan: "Upregulation of the tissue inhibitor of metalloproteinase-1 protein is associated with progression of human non-small-cell lung cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 22, Issue 16, pp. 3218-29, 2004

Aljada, Ramnath, Donohue, Harvey, Brooks, Wiseman, Khoury, Loewen, Slocum, Anderson, Bepler, Tan: "Upregulation of the tissue inhibitor of metalloproteinase-1 protein is associated with progression of human non-small-cell lung cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 22, Issue 16, pp. 3218-29, 2004 (PubMed).

Hornebeck: "Down-regulation of tissue inhibitor of matrix metalloprotease-1 (TIMP-1) in aged human skin contributes to matrix degradation and impaired cell growth and survival." in: Pathologie-biologie, Vol. 51, Issue 10, pp. 569-73, 2003 (PubMed).

Gardner, Ghorpade: "Tissue inhibitor of metalloproteinase (TIMP)-1: the TIMPed balance of matrix metalloproteinases in the central nervous system." in: Journal of neuroscience research, Vol. 74, Issue 6, pp. 801-6, 2003 (PubMed).

Osthues, Knäuper, Oberhoff, Reinke, Tschesche: "Isolation and characterization of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) from human rheumatoid synovial fluid." in: FEBS letters, Vol. 296, Issue 1, pp. 16-20, 1992 (PubMed).

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