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IgE protein (Biotin)

ABIN1583010 产品详细信息, 供应商: Log in to see
蛋白名称
  • IGH2
  • IgE
  • Gm900
  • immunoglobulin heavy chain (epsilon polypeptide)
  • immunoglobulin heavy constant epsilon
  • Immunoglobulin heavy constant epsilon
  • Ighe
  • IGHE
宿主

29
6
1
资源
Human
29
6
1
克隆类型
多克隆
标记
Biotin
应用范围
Isotype Control (IsoC), ELISA
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产品特性 This product is purified human IgE with kappa light chains produced in vitro from a hybridoma. Plasma IgE concentrations are low in normal patients. Elevated IgE levels are found in patients presenting severe allergic reactions and parasite infections. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is > 98 % by SDS-PAGE analysis. There is no contamination by antibodies of other isotypes because of the cell line origin. Contaminants from foetal bovine serum were removed by Protein-L affinity chromatography. The material must be handled as potentially infectious as all human material. The product can be used as a control.
This is a non-immune hybridoma.
纯化方法 Affinity Chromatography on Protein L
纯度 > 98 % by SDS-PAGE
研究领域 Immunology, Secondary Antibodies
应用备注 Purified human IgE with kappa light chain is produced in vitro from a monoclonal hybridoma. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests.
限制 仅限研究用
状态 Liquid
浓度 1 mg/mL
缓冲液 0.1 mg IgE in 0.1 mL of PBS pH 7.4, 0.1 % BSA with 0.09 % sodium azide.
储存液 Sodium azide
注意事项 Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
注意事项 Minimize freeze-thaw cycles. The material must be handled as potentially infectious as all human material.
储存条件 -20 °C
有效期 12 months
厂商提供的图像
 image for IgE protein (Biotin) (ABIN1583010) Quality control of human IgE preparation from hybridoma (lane 2) vs. IgE sample purif...
ELISA image for IgE protein (Biotin) (ABIN1583010) IgE protein (Biotin) (Image 2)
有引用在: Serra-Pages, Olivera, Torres, Picado, de Mora, Rivera: "E-prostanoid 2 receptors dampen mast cell degranulation via cAMP/PKA-mediated suppression of IgE-dependent signaling." in: Journal of leukocyte biology, Vol. 92, Issue 6, pp. 1155-65, 2012 (PubMed).

Ainsua-Enrich, Alvarez-Errico, Gilfillan, Picado, Sayós, Rivera, Martín: "The adaptor 3BP2 is required for early and late events in FcεRI signaling in human mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 2727-34, 2012 (PubMed).

Janson, Kasahara, Prendergast, Colicelli: "RIN3 is a negative regulator of mast cell responses to SCF." in: PLoS ONE, Vol. 7, Issue 11, pp. e49615, 2012 (PubMed).

Álvarez-Errico, Oliver-Vila, Ainsua-Enrich, Gilfillan, Picado, Sayós, Martín: "CD84 negatively regulates IgE high-affinity receptor signaling in human mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 5577-86, 2011 (PubMed).

Charles, Hardwick, Daugas, Illei, Rivera: "Basophils and the T helper 2 environment can promote the development of lupus nephritis." in: Nature medicine, Vol. 16, Issue 6, pp. 701-7, 2010 (PubMed).