IL-10 蛋白
(4 references)-
- 抗原 See all IL-10 (IL10) 蛋白
- IL-10 (IL10) (Interleukin 10 (IL10))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: D36 indicator cells were co-stimulated with recombinant mouse IL-4 (Cat No. 550067) at 50 pg/mL to promote proliferation and were incubated for 24 hours prior to being pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 1.0 - 6.6 x 10^5 ng/mL
ED50: 0.5 - 100 ng/mL - 产品特性
- The recombinant mouse IL-10 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and containing 4 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Mouse IL-10 was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
- 纯化方法
- Recombinant mouse IL-10 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-10 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of mouse IL-10 as measured in chromogenic LAL assay.
- Top Product
- Discover our top product IL10 蛋白
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Interleukin 10 (IL10) (AA 19-178) protein
IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 10 (IL10) protein (His-Avi Tag)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 10 (IL10) protein (His-Avi Tag,Biotin)IL10 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Interleukin 10 (IL10) (AA 19-178) (Active) proteinIL10 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 10 (IL10) (Active) proteinIL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 10 (IL10) (AA 22-178) protein (His tag)IL10 宿主: Hamster 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Interleukin 10 (IL10) (AA 1-178) protein (GST tag)IL10 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 10 (IL10) (AA 74-178) protein (GST tag)IL10 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 10 (IL10) proteinIL10 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Interleukin 10 (IL10) (AA 19-178) protein (His tag)Crystallography grade IL10 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- ELISA Standard: Mouse IL-10 is useful as a quantitative standard for measuring mouse IL-10 protein levels in an IL-10 specific sandwich ELISA with the purified JES5-2A5 antibody (Cat. No. 551215) as a capture antibody and the biotinylated clone SXC-1 (Cat. No. 554423) or JES-16E3 (Cat. No. 554465) as the detection antibody. To obtain linear standard curves, doubling dilutions of the mouse IL-10 standard from ~2,000 to 15 pg/mL should be included with each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring IL-10 in serum or plasma, the BD OptEIA™ Mouse IL-10 Set (Cat. No. 555252) is specially formulated and recommended.
- 说明
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BD Pharmingen™ Recombinant Mouse IL-10 - Reactivity Ms
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA and glycerol.
- 储存液
- Azide free
- 注意事项
- Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of pro-inflammatory T cell mediated immunity. Mouse IL-10 is an 18.8 kD protein containing 160 amino acid residues.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).
: "Biological properties of interleukin 10." in: Journal of clinical immunology, Vol. 12, Issue 4, pp. 239-47, (1992) (PubMed).
: "Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI." in: Science (New York, N.Y.), Vol. 248, Issue 4960, pp. 1230-4, (1990) (PubMed).
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: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).
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- 抗原
- IL-10 (IL10) (Interleukin 10 (IL10))
- 别名
- IL-10 (IL10 产品)
- 别名
- CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein
- 背景
- Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of pro-inflammatory T cell mediated immunity. Mouse IL-10 is an 18.8 kD protein containing 160 amino acid residues.
- 途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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