(1 reference)-
- 抗原 See all LTA 蛋白
- LTA (Lymphotoxin-alpha (LTA))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- ELISA, Functional Studies (Func)
- 品牌
- BD Pharmingen™
- 特异性
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Biological Activity: Bioactivity is tested in MTT/actinomycin D cytolysis assay using L929 indicator cells.
Specific Activity Range: 0.3 - 5.0x10^9 Unit /mg
ED50: 2 - 30 pg/ml
Observed dose response: > 500 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. - 产品特性
- Recombinant human LT-alpha is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco’s PBS (pH 7.2) and containing biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant human LT-alpha is >95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.
- 纯化方法
- Recombinant human LT-α is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant human LT-α is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.
- 纯度
- ≥ 95 %
- 过滤
- 0.22 μm filtered
- 内毒素水平
- The endotoxin level is ≤ 0.1 ng per µg of LT-alpha, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product LTA 蛋白
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Lymphotoxin-alpha (LTA) (Active) protein
LTA 宿主: 化学剂 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Lymphotoxin-alpha (LTA) (AA 58-205) protein (His-DYKDDDDK-Strep II Tag)LTA 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Lymphotoxin-alpha (LTA) (AA 34-202) protein (His tag)LTA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lymphotoxin-alpha (LTA) (AA 35-205) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lymphotoxin-alpha (LTA) (AA 35-205) (Active) protein (His tag)LTA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Lymphotoxin-alpha (LTA) (AA 1-205) protein (GST tag)LTA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Lymphotoxin-alpha (LTA) (AA 106-205) protein (GST tag)LTA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 应用备注
- ELISA Standard: This recombinant human LT-alpha is useful as a quantitative standard for measuring human LT-alpha protein levels in a LT-alpha specific sandwich ELISA with the purified 359-238-8 antibody (Cat. No. 551222) as a capture antibody and the biotinylated 359-81-11 antibody (Cat. No. 554555) as the detection antibody.
- 说明
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BD Pharmingen™ Recombinant Human LT-α - Reactivity Hu
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- Frozen aqueous buffered solution containing BSA.
- 注意事项
- Lymphotoxin (LT-α), also known as, Tumor Necrosis Factor-β (TNF-β) is a potent multifunctional cytokine which is primarily produced by activated lymphocytes. LT-α has cytotoxic activity against a wide range of tumor cells and certain other target cells. Other LT-α activities include the activation of neutrophils, osteoclasts, and endothelial cells and the upregulation of the membrane expression of MHC antigens and adhesion molecules by various cell types. Human LT-α is a 18 kDa protein containing 171 amino acid residues.
- 储存条件
- -80 °C
- 储存方法
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "Lymphotoxin." in: Annual review of immunology, Vol. 6, pp. 407-38, (1988) (PubMed).
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: "Lymphotoxin." in: Annual review of immunology, Vol. 6, pp. 407-38, (1988) (PubMed).
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- 抗原
- LTA (Lymphotoxin-alpha (LTA))
- 别名
- LT-alpha (LTA 产品)
- 别名
- LT Protein, TNFB Protein, TNFSF1 Protein, LT-[a] Protein, LT-alpha Protein, LT[a] Protein, LTalpha Protein, Ltx Protein, TNF-beta Protein, Tnfb Protein, Tnfsf1b Protein, hlb382 Protein, lymphotoxin alpha Protein, lymphotoxin A Protein, LTA Protein, Lta Protein
- 物质类
- Chemical
- 背景
- Lymphotoxin (LT-α), also known as, Tumor Necrosis Factor-β (TNF-β) is a potent multifunctional cytokine which is primarily produced by activated lymphocytes. LT-α has cytotoxic activity against a wide range of tumor cells and certain other target cells. Other LT-α activities include the activation of neutrophils, osteoclasts, and endothelial cells and the upregulation of the membrane expression of MHC antigens and adhesion molecules by various cell types. Human LT-α is a 18 kDa protein containing 171 amino acid residues.
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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