Recombinant Human Tumor Necrosis Factor beta/TNF-beta is produced by our E. coli expression system. The target protein is expressed with sequence (Leu35-Leu205) of Human TNF-beta.
纯度
> 95 % as determined by reducing SDS-PAGE.
过滤
0.2 μm filtered
内毒素水平
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
LTA
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
SDS, Func
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
缓冲液
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
注意事项
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
储存条件
4 °C/-20 °C/-80 °C
储存方法
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Tumor Necrosis Factor beta (TNF-beta) is a secreted protein belonging to the tumor necrosis factor family. TNF-beta binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM in homotrimeric form, binds to TNFRSF3/LTBR in heterotrimeric form with LTB. TNF-beta forms heterotrimers with lymphotoxin-beta, which anchors TNF-beta to the cell surface. TNF-beta mediates the inflammatory, immunostimulatory, and antiviral response, involves in the formation of second lymphoid organs during development, has a role in apoptosis. TNF-beta is produced by lymphocytes and cytotoxic for a variety of tumor cells in vitro and in vivo. Alternative Names: Lymphotoxin-Alpha, LT-Alpha, TNF-Beta, Tumor Necrosis Factor Ligand Superfamily Member 1, LTA, TNFB, TNFSF1