Protein phosphatase 1 (AA 1-147), (Regulatory Subunit 14A) protein (His tag)
宿主: 人
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- 抗原
- Protein phosphatase 1
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-147, Regulatory Subunit 14A
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 原理
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A/PPP1R14A (C-6His)
- 序列
- MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHPLEH HHHHH
- 产品特性
- Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A/PPP1R14A is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Pro147) of Human PPP1R14A.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Protein phosphatase 1 (Regulatory Subunit 15A) protein
宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, WB, ELISA, IP
Protein phosphatase 1 (Regulatory Subunit 15A) protein宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, WB, ELISA, IP
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 0.2 mM EDTA, 1 mM DTT, 10 % Glycerol, pH 8.0.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- Protein phosphatase 1
- 别名
- Ppp1cb Protein, protein phosphatase 1 catalytic subunit beta Protein, PPP1CB Protein
- 背景
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Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime, PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated, PPP1R14A has over 1000-fold higher inhibitory activity, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition, inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.
Alternative Names: Protein Phosphatase 1 Regulatory Subunit 14A, 17 kDa PKC-Potentiated Inhibitory Protein of PP1, Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa, CPI-17, PPP1R14A, CPI17, PPP1INL - 分子量
- 17.76 kDa
- UniProt
- Q96A00
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