PKC epsilon Protein (AA 580-737) (His tag)
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- 抗原 See all PKC epsilon (PRKCE) 蛋白
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 蛋白类型
- Recombinant
- 产品特性
- AA 580-737
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PKC epsilon protein is labelled with His tag.
- 原理
- Recombinant Human Protein Kinase C Type ε/PKC ε/PKCE (C-6His)
- 序列
- MQELEYGPSV DWWALGVLMY EMMAGQPPFE ADNEDDLFES ILHDDVLYPV WLSKEAVSIL KAFMTKNPHK RLGCVASQNG EDAIKQHPFF KEIDWVLLEQ KKIKPPFKPR IKTKRDVNNF DQDFTREEPV LTLVDEAIVK QINQEEFKGF SYFGEDLMPL EHHHHHH
- 产品特性
- Recombinant Human PKC epsilon is produced with our E. coli expression system. The target protein is expressed with sequence (Gln580-Pro737) of Human PRKCE.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PRKCE 蛋白
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Protein Kinase C, epsilon (PRKCE) (AA 1-737) (Active) protein (His-GST)
PRKCE 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Protein Kinase C, epsilon (PRKCE) (AA 580-737) protein (His tag)PRKCE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (Strep Tag)Crystallography grade PRKCE 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase C, epsilon (PRKCE) (AA 1-737) protein (Strep Tag)Crystallography grade PRKCE 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.
- 储存液
- Dithiothreitol (DTT)
- 注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))
- 别名
- PKC epsilon (PRKCE 产品)
- 别名
- PKCE Protein, nPKC-epsilon Protein, 5830406C15Rik Protein, PKC[e] Protein, PKCepsilon Protein, Pkce Protein, R75156 Protein, fj59b07 Protein, prkce Protein, wu:fj59b07 Protein, pkce Protein, pkc-epsilon Protein, PRKCH Protein, protein kinase C epsilon Protein, protein kinase C, epsilon Protein, protein kinase C, epsilon a Protein, PRKCE Protein, Prkce Protein, prkcea Protein, prkce Protein
- 背景
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Protein Kinase C Epsilon type is a member of the serine- and threonine-specific protein kinase family that can be activated by calcium and the second messenger diacylglycerol. Protein Kinase C Epsilon contains these domains: one AGC-kinase C-terminal domain, one C2 domain, one protein kinase domain and two phorbol-ester/DAG-type zinc fingers. Protein Kinase C Epsilon phosphorylate a variety of protein targets and has been identified to participate in diverse cellular signaling pathways. It has many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Protein Kinase C Epsilon also serves as the receptor for phorbol esters, a class of tumor promoters.ReferencesZhang KL, et al. Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas.PMID: 24012640http://www.ncbi.nlm.nih.gov/pubmed/24012640
Alternative Names: Protein Kinase C Epsilon Type, nPKC-Epsilon, PRKCE, PKCE - 分子量
- 19.64 kDa
- UniProt
- Q02156
- 途径
- TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling
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