MDH1 Protein (AA 2-334) (His tag)
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- 抗原 See all MDH1 蛋白
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-334
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MDH1 protein is labelled with His tag.
- 原理
- Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 (C-6His)
- 序列
- SEPIRVLVTG AAGQIAYSLL YSIGNGSVFG KDQPIILVLL DITPMMGVLD GVLMELQDCA LPLLKDVIAT DKEDVAFKDL DVAILVGSMP RREGMERKDL LKANVKIFKS QGAALDKYAK KSVKVIVVGN PANTNCLTAS KSAPSIPKEN FSCLTRLDHN RAKAQIALKL GVTANDVKNV IIWGNHSSTQ YPDVNHAKVK LQGKEVGVYE ALKDDSWLKG EFVTTVQQRG AAVIKARKLS SAMSAAKAIC DHVRDIWFGT PEGEFVSMGV ISDGNSYGVP DDLLYSFPVV IKNKTWKFVE GLPINDFSRE KMDLTAKELT EEKESAFEFL SSALEHHHHH H
- 产品特性
- Recombinant Human Malate Dehydrogenase, Cytoplasmic/MDH1 is produced by our E. coli expression system. The target protein is expressed with sequence (Ser2-Ala334) of Human MDH1 fused with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product MDH1 蛋白
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Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) protein (His tag)
MDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) protein (GST tag)MDH1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 101-193) protein (GST tag)MDH1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 1-334) (Active) protein (His tag)MDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) protein (His tag)MDH1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by SDS-PAGE
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-334) protein (His tag)Crystallography grade MDH1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (AA 2-333), (full length) protein (GST tag)MDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- 储存条件
- -80 °C
- 储存方法
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - 有效期
- 6 months
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- 抗原
- MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))
- 别名
- MDH1 (MDH1 产品)
- 别名
- MDH-s Protein, MDHA Protein, MGC:1375 Protein, MOR2 Protein, ODS1 Protein, B230377B03Rik Protein, D17921 Protein, Mor-2 Protein, Mor2 Protein, MDL1 Protein, Mdhl Protein, MDH Protein, mdh-s Protein, mdha Protein, mor2 Protein, MDH-1 Protein, Mdh Protein, Mdh-1 Protein, Mdh2 Protein, MdhD Protein, cMdh Protein, sMdh Protein, malate dehydrogenase 1 Protein, malic enzyme 2 Protein, malate dehydrogenase 1, NAD (soluble) Protein, malate dehydrogenase 1 S homeolog Protein, malate dehydrogenase, cytoplasmic Protein, Malate dehydrogenase 1 Protein, MDH1 Protein, ME2 Protein, Mdh1 Protein, mdh1.S Protein, LOC100280767 Protein
- Sub Type
- Fusionprotein
- 背景
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Malate Dehydrogenase, Cytoplasmic (MDH1) is an enzyme which belongs to the MDH Type 2 sub-family of LDH/MDH superfamily. MDH1 is involved in the Citric Acid Cycle that catalyzes the conversion of Malate into Oxaloacetate (using NAD+) and vice versa. MDH1 should not be confused with Malic Enzyme, which catalyzes the conversion of Malate to Pyruvate, producing NADPH. MDH1 also participates in Gluconeogenesis, the synthesis of Glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by Pyruvate Carboxylase to form Pxaloacetate, a Citric Acid Cycle intermediate. In order to transport the Oxaloacetate out of the Mitochondria, Malate Dehydrogenase reduces it to Malate, and it then traverses the inner Mitochondrial membrane. Once in the cytosol, the Malate is oxidized back to Oxaloacetate by MDH1. Finally, Phosphoenol-Pyruvate Carboxy Kinase (PEPCK) converts Oxaloacetate to Phosphoenol Pyruvate.
Alternative Names: Malate Dehydrogenase Cytoplasmic, Cytosolic Malate Dehydrogenase, Diiodophenylpyruvate Reductase, MDH1, MDHA - 分子量
- 37.5 kDa
- UniProt
- P40925
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