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Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag)

ABIN1047668 产品详细信息, 供应商: Log in to see
蛋白名称
  • hm:zeh0062
  • AA517111
  • BM-40
  • ON
  • SPARC
  • secreted acidic cysteine rich glycoprotein
  • secreted protein, acidic, cysteine-rich (osteonectin)
  • SPARC protein
  • SPARC-like
  • sparc
  • Sparc
  • SPARC
  • MGC64258
  • LOC100855736
产品特性
AA 18-303, full length
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1
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宿主

24
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资源
大肠杆菌(E. Coli)
15
7
3
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2
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2
1
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1
蛋白类型
Recombinant
标记
This SPARC protein is labelled with GST tag.
应用范围
ELISA
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序列 APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
产品特性 Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
纯度 90 %
背景 Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites, an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.
分子量 60.1 kD
UniProt P09486
途径 Autophagy
说明

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

限制 仅限研究用
状态 Lyophilized
浓度 0.2-2 mg/mL
缓冲液 Tris-based buffer, 50 % glycerol
注意事项 Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
储存条件 -20 °C
储存方法 Store at -20 °C for extended storage, conserve at -20 °C or -80 °C
厂商提供的图像
SDS-PAGE (SDS) image for Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full length) protein (GST tag) (ABIN1047668) Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303), (full leng...
背景 Lankat-Buttgereit, Mann, Deutzmann, Timpl, Krieg: "Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin)." in: FEBS letters, Vol. 236, Issue 2, pp. 352-6, 1988 (PubMed).