anti-人 MDM2 抗体 for Immunoprecipitation

Recommended MDM2 Antibody (supplied by: Log in to see )

抗原
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) 抗体
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
抗原表位
Center
46
42
36
29
26
24
15
15
15
12
9
8
7
7
6
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
378
135
95
14
8
6
4
2
2
2
2
2
2
1
1
1
宿主

305
77
14
2
克隆类型
多克隆
标记
This MDM2 antibody is un-conjugated
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
应用范围
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
262
136
108
83
71
40
38
24
12
12
12
12
4
4
2
1
1
1
1
1
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产品编号 ABIN2854782
10,544.15 ¥
(含税, 含运费)
来自4A Biotech Co.,Ltd.(本地经销商)
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967511 BI IHC (f) IHC (fro) IP WB Mouse IgG1 AA 154-167 Log in to see SMP14 2
1 ABIN180331 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 154-167 Log in to see SMP14 3
1 ABIN2957662 IC IF IP IHC WB Rabbit Log in to see Polyclonal
1 ABIN214211 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN2469738 IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN2475548 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Log in to see SMP14 3
1 ABIN2706519 IC IF IP IHC WB Rabbit pSer166 Log in to see Polyclonal
1 ABIN1724157 IHC (fro) IHC (p) IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN1493454 IP Sepharose Rabbit AA 418-432 Log in to see Polyclonal
1 ABIN1495411 IP Magnetic Particles Rabbit AA 103-119 Log in to see Polyclonal
1 ABIN4904328 IF IHC IP WB Mouse IgG Log in to see
1 ABIN216012 IHC (fro) IF IHC (p) IP WB Mouse IgG2b, kappa AA 26-169 Log in to see
1 ABIN2312928 IC IF IP IHC WB Mouse IgG2b kappa AA 26-169 Log in to see 3G187
1 ABIN2625321 ICC IF IHC (p) IP WB Rabbit pSer166 Log in to see Polyclonal
1 ABIN2312952 IP IHC WB Mouse IgG1 AA 154-167 Log in to see 3H2649
1 ABIN5620135 ICC IF IHC IP WB Rabbit Center Log in to see Polyclonal
1 ABIN2313024 IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN5583358 IHC (fro) IHC (p) IP Mouse IgG1 Log in to see SMP14
1 ABIN643838 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 Log in to see
1 ABIN2767770 IHC (fro) IP IHC (p) IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14

General

抗原 Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) 抗体
抗原表位 Center
(46), (42), (36), (29), (26), (24), (15), (15), (15), (12), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(378), (135), (95), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
宿主
(305), (77), (14), (2)
克隆类型
多克隆
标记 This MDM2 antibody is un-conjugated
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
应用范围 Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(262), (136), (108), (83), (71), (40), (38), (24), (12), (12), (12), (12), (4), (4), (2), (1), (1), (1), (1), (1)
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MDM2抗体详情

MDM2目标详情 使用细节 贮存及处理 图像
交叉反应 猫, 犬, 猪
交叉反应 (详细) Cat (95 %), Dog (91 %), Pig (93 %)
产品特性 Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
MDM2 antibody
纯化方法 Purified by antigen-affinity chromatography.
免疫原 Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
亚型 IgG

MDM2目标详情

MDM2抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 MDM2 (MDM2 Antibody 摘要)
背景 This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
分子量 55 kDa
基因ID 4193
研究领域 Cancer, Cell Cycle, Transcription Factors
途径 p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy

使用细节

MDM2抗体详情 MDM2目标详情 贮存及处理 图像 回页首
应用备注 Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
说明

Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T

限制 仅限研究用

贮存及处理

MDM2抗体详情 MDM2目标详情 使用细节 图像 回页首
状态 Liquid
浓度 0.74 mg/mL
缓冲液 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
储存液 Thimerosal (Merthiolate)
注意事项 This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

图像

MDM2抗体详情 MDM2目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T w...
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2, ...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg Neuro2...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg H1299 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ChIP Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of ei...
Immunoprecipitation (IP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples:...