|抗原||E2F Transcription Factor 1 (E2F1) 抗体|
|抗原表位||AA 360-369, pSer364 其他选择|
|适用||Chimpanzee, 人 其他选择|
|标记||This E2F1 antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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E2F1抗体详情E2F1目标详情 使用细节 贮存及处理 图像
|特异性||This antibody is directed against the phosphorylated form of E2F-1 at the pS364 residue. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites.|
Species reactivity (expected):Chimpanzee 100 % .
Species reactivity (tested):Human.
|产品特性||Concentration: 0.80 mg/mL (by UV absorbance at 280 nm)|
|纯化方法||The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.|
|免疫原||Synthetic peptide corresponding to amino acids 360-369 of Human E2F-1|
E2F1目标详情E2F1抗体详情 使用细节 贮存及处理 图像 回页首
|别名||E2F1 (E2F1 Antibody 摘要)|
|背景||E2F-1 (also known as tran-scription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1|
|研究领域||Cancer, Transcription Factors|
|途径||p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy|
使用细节E2F1抗体详情 E2F1目标详情 贮存及处理 图像 回页首
ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 250 - 1: 2,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理E2F1抗体详情 E2F1目标详情 使用细节 图像 回页首
|缓冲液||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|