anti-Chimpanzee E2F1 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended E2F1 Antibody (supplied by: Log in to see )

抗原
E2F Transcription Factor 1 (E2F1) 抗体
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
抗原表位
AA 360-369, pSer364
44
36
31
27
22
21
17
15
14
14
12
11
11
11
10
9
9
8
8
7
5
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
Chimpanzee, 人
425
125
47
10
8
6
6
5
5
4
3
3
1
1
1
宿主

287
126
21
1
克隆类型
多克隆
标记
This E2F1 antibody is un-conjugated
25
23
20
15
13
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
337
181
111
86
78
58
56
42
39
28
8
3
2
2
1
1
1
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产品编号 ABIN5551744
$ 411.18
加上运输费 $45.00

General

抗原 E2F Transcription Factor 1 (E2F1) 抗体
抗原表位 AA 360-369, pSer364
(44), (36), (31), (27), (22), (21), (17), (15), (14), (14), (12), (11), (11), (11), (10), (9), (9), (8), (8), (7), (5), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 Chimpanzee, 人
(425), (125), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (1), (1), (1)
宿主
(287), (126), (21), (1)
克隆类型
多克隆
标记 This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (13), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(337), (181), (111), (86), (78), (58), (56), (42), (39), (28), (8), (3), (2), (2), (1), (1), (1)
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E2F1抗体详情

E2F1目标详情 使用细节 贮存及处理 图像
特异性 This antibody is directed against the phosphorylated form of E2F-1 at the pS364 residue. Reactivity with non-phosphorylated human E2F-1 is minimal by ELISA. The antibody does not cross-react with E2F-1 phosphorylated at other sites.
交叉反应 (详细) Species reactivity (expected):Chimpanzee 100 % .
Species reactivity (tested):Human.
产品特性 Concentration: 0.80 mg/mL (by UV absorbance at 280 nm)
纯化方法 The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
免疫原 Synthetic peptide corresponding to amino acids 360-369 of Human E2F-1
亚型 IgG

E2F1目标详情

E2F1抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 E2F1 (E2F1 Antibody 摘要)
背景 E2F-1 (also known as tran-scription factor E2F-1, Retinoblastoma binding protein 3, RBBP-3, PRB-binding protein E2F-1, PBR3, Retinoblastoma-associated protein 1 and RBAP-1) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several domains con-served through evolution that are found in most members of the family. These domains include a DNA binding domain, a dimerization domain that determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein as well as E2F2 and E2F3 have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner and mediates both cell proliferation and p53-dependent/independent apoptosis. Increased nuclear expression of this protein has been reported in a variety of cancers.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
基因ID 1869
NCBI登录号 NP_005216
UniProt Q01094
研究领域 Cancer, Transcription Factors
途径 p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

使用细节

E2F1抗体详情 E2F1目标详情 贮存及处理 图像 回页首
应用备注 ELISA: 1: 20,000 - 1: 100,000. Western blot: 1: 250 - 1: 2,000. Immunohistochemistry on paraffin sections: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

E2F1抗体详情 E2F1目标详情 使用细节 图像 回页首
浓度 0.80 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.