anti-人 Tumor Necrosis Factor 抗体 for Flow Cytometry

Recommended Tumor Necrosis Factor Antibody (supplied by: Log in to see )

抗原
Tumor Necrosis Factor (TNF) 抗体
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
抗原表位
AA 77-233
43
39
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
适用

1229
445
358
145
136
119
119
62
55
41
29
18
13
12
9
7
6
4
4
4
4
2
1
1
1
1
宿主
小鼠
928
490
182
48
10
9
7
6
3
2
2
克隆类型
单克隆
标记
This Tumor Necrosis Factor antibody is un-conjugated
165
97
71
50
32
30
27
26
25
25
25
25
25
25
24
24
24
20
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
应用范围
Cell Culture (CC), ELISA, Flow Cytometry (FACS)
869
853
599
311
273
230
202
181
137
118
98
70
27
26
25
22
20
16
14
8
7
6
6
6
6
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

产品编号 ABIN1997163
产品在该地区无法提供。
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.368205 ABIN263070 ICC FACS IF IHC (p) FITC Mouse IgG1 kappa Log in to see MAb11 1
14.368205 ABIN263068 ELISA FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see MAb11 1
14.368205 ABIN1383957 FACS PE Mouse IgG1 Log in to see B-D9 1
14.368205 ABIN4898909 FACS Fluorescein Mouse IgG1 Log in to see 6402 4
14.368205 ABIN4898910 FACS PE Mouse IgG1 Log in to see 6402 7
14.368205 ABIN1383956 FACS FITC Mouse IgG1 Log in to see B-D9 1
14.368205 ABIN2662374 FACS Pacific Blue Mouse IgG1 kappa Log in to see MAb11 11
14.368205 ABIN2658839 FACS APC Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2660503 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2660627 FACS APC-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2663940 FACS PE Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2659787 BR FACS IF IHC PE/Dazzle™ 594 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2657587 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2657286 FACS Alexa Fluor 700 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN2662036 FACS FITC Mouse IgG1 kappa Log in to see MAb11 11
14.368205 ABIN2659610 FACS PE-Cy7 Mouse IgG1 kappa Log in to see MAb11 10
14.368205 ABIN1997166 FACS FITC Mouse IgG1 AA 77-233 Log in to see 01
14.368205 ABIN1997168 FACS FITC Rabbit IgG AA 77-233 Log in to see 101
14.368205 ABIN5503748 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401
14.368205 ABIN5503749 FACS Fluorescein Mouse IgG1 AA 57-233, Membrane Form Log in to see 6401

引用最多的anti-Tumor Necrosis Factor 抗体 for Flow Cytometry

Similar anti-Tumor Necrosis Factor 抗体

应用范围 / 适用
Activity Inhibition (Act Inh) 2 抗体
Biochemical Assay (BCA) 1 抗体
Blocking Antibody (Inhibition) 13 抗体
Blocking Peptide (BP) 5 抗体
Blocking Reagent (BR) 5 抗体
Cell Culture (CC) 1 抗体
Cellular Assay (CA) 2 抗体
Cytometry by Time of Flight (CyTOF) 27 抗体
Dot Blot (DB) 2 抗体
ELISA 580 抗体
ELISA (Capture) 5 抗体
ELISA (Detection) 4 抗体
ELISpot 9 抗体
ELISpot (Capture) (ELISPOT (Capture)) 1 抗体
ELISpot (Detection) (ELISPOT (Detection)) 1 抗体
Enzyme Immunoassay (EIA) 49 抗体
Flow Cytometry (FACS) 505 抗体
Functional Studies (Func) 68 抗体
Immunoassay (IA) 13 抗体

General

抗原 Tumor Necrosis Factor (TNF) 抗体
抗原表位 AA 77-233
(43), (39), (29), (27), (24), (22), (17), (17), (15), (11), (11), (10), (10), (8), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(1229), (445), (358), (145), (136), (119), (119), (62), (55), (41), (29), (18), (13), (12), (9), (7), (6), (4), (4), (4), (4), (2), (1), (1), (1), (1)
宿主 小鼠
(928), (490), (182), (48), (10), (9), (7), (6), (3), (2), (2)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Tumor Necrosis Factor antibody is un-conjugated
(165), (97), (71), (50), (32), (30), (27), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (20), (13), (9), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
应用范围 Cell Culture (CC), ELISA, Flow Cytometry (FACS)
(869), (853), (599), (311), (273), (230), (202), (181), (137), (118), (98), (70), (27), (26), (25), (22), (20), (16), (14), (8), (7), (6), (6), (6), (6), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 找到1个引用
Supplier Log in to see

Tumor Necrosis Factor抗体详情

Tumor Necrosis Factor目标详情 使用细节 贮存及处理 ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) 图像
特异性 Human TNFα
No cross-reactivity in WB and ELISA with
E.coli cell lysate
无交叉反应 大肠杆菌(E. Coli)
产品特性 This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα, ABIN2003122 Val 77-Leu 233, NP_000585.2,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
纯化方法 purified by Protein A affinity chromatography
过滤 0.2 μm filtered
免疫原 Recombinant Human TNFalpha protein (ABIN2003122)
克隆位点 9A7A5G9
亚型 IgG1

Tumor Necrosis Factor目标详情

Tumor Necrosis Factor抗体详情 使用细节 贮存及处理 ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) 图像 回页首
抗原
别名 TNF (TNF Antibody 摘要)
背景 Tumor necrosis factor alpha (TNFα), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily. It is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFα is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). Two receptors mediating the TNFα function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. TNFα has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.
NCBI登录号 NP_000585.2
途径 NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

使用细节

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 贮存及处理 ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) 图像 回页首
应用备注 ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TNFα. The detection limit for Human TNFα is approximately 0.6 ng/well.
ELISA(Cap): 0.5-4 μg/mL
FCM: 0.5-2 μg/Test
限制 仅限研究用

贮存及处理

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) 图像 回页首
状态 Liquid
缓冲液 0.2 μm filtered solution in PBS
储存液 Without preservative
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 4 °C,-20 °C,-80 °C
储存方法 This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
有效期 12 months

ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163)

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Cohen, Sabhachandani, Golberg, Konry: "Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detection." in: Biosensors & bioelectronics, Vol. 66, pp. 454-60, 2014

图像

Tumor Necrosis Factor抗体详情 Tumor Necrosis Factor目标详情 使用细节 贮存及处理 ProductDetails: References for anti-Tumor Necrosis Factor antibody (ABIN1997163) 回页首
厂商提供的图像
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor 抗体 (TNF) (AA 77-233) (ABIN1997163) Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and sti...