anti-人 EGF 抗体 for Immunohistochemistry (Frozen Sections)

Recommended EGF Antibody (supplied by: Log in to see )

抗原
Epidermal Growth Factor (EGF) 抗体
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
  • LOC100008808
适用

339
164
87
19
14
7
4
2
2
1
1
1
宿主

308
153
29
24
6
4
克隆类型
多克隆
标记
This EGF antibody is conjugated to Biotin
46
27
22
12
10
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
353
290
129
91
54
33
30
27
26
25
19
13
10
7
6
5
4
3
2
2
2
1
1
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产品编号 ABIN115992
4,426.81 ¥
(含税, 含运费)
来自4A Biotech Co.,Ltd.(本地经销商)
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115991 EIA IHC (fro) IHC (p) WB Biotin Rabbit Log in to see Polyclonal
1 ABIN115993 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN115994 EIA Func IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN3201442 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG AA 609-751 Log in to see
1 ABIN4959675 ELISA IHC IHC (fro) IHC (p) WB Mouse IgM Log in to see F5

General

抗原 Epidermal Growth Factor (EGF) 抗体
适用
(339), (164), (87), (19), (14), (7), (4), (2), (2), (1), (1), (1)
宿主
(308), (153), (29), (24), (6), (4)
克隆类型
多克隆
标记 This EGF antibody is conjugated to Biotin
(46), (27), (22), (12), (10), (8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(353), (290), (129), (91), (54), (33), (30), (27), (26), (25), (19), (13), (10), (7), (6), (5), (4), (3), (2), (2), (2), (1), (1)
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EGF抗体详情

EGF目标详情 使用细节 贮存及处理 图像
特异性 This antibody reacts with Epidermal Growth Factor (hEGF).
纯化方法 Affinity chromatography.
免疫原 Highly pure (>98%) recombinant hEGF (human EGF).

EGF目标详情

EGF抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 EGF (EGF Antibody 摘要)
背景 Epidermal growth factor (EGF) has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells. The EGF precursor is believed to exist as a membrane bound molecule which is proteolytically cleaved to generate the 53 amino acid peptide hormone that stimulates cells to divide. EGF exerts its actions by binding to the EGFR, a 170 kDa protein. Epidermal growth factor (EGF) is a key growth factor regulating cell survival. Through its binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of the PI3K/AKT, RAS/ERK and JAK/STAT pathways. Because of its key role in driving the proliferation of cells, EGFR is a target of several anti-cancer drugs currently in development.Synonyms: Epidermal growth factor, HOMG4, Pro-epidermal growth factor, URG, Urogastrone
基因ID 1950
NCBI登录号 NP_001954
UniProt P01133
研究领域 Cancer, Growth Factors
途径 NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation

使用细节

EGF抗体详情 EGF目标详情 贮存及处理 图像 回页首
应用备注 ELISA: To detect hEGF by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEGF is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

EGF抗体详情 EGF目标详情 使用细节 图像 回页首
溶解方式 Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
缓冲液 PBS, pH 7.2 without preservatives.
储存液 Without preservative
注意事项 Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
储存条件 4 °C/-20 °C
储存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.