anti-人 CD40 抗体 for Immunofluorescence

Recommended CD40 Antibody (supplied by: Log in to see )

抗原
CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) 抗体
  • Bp50
  • CDW40
  • TNFRSF5
  • p50
  • 2310042E05Rik
  • C130077D06Rik
  • C87049
  • DCTN-50
  • GMP23-48K
  • RBP50
  • AI326936
  • GP39
  • HIGM1
  • IGM
  • IMD3
  • T-BAM
  • TRAP
  • Tnfrsf5
  • TNFSF5
  • CD40 molecule
  • dynactin 2
  • CD40 antigen
  • CD40
  • Dctn2
  • Cd40
抗原表位
AA 71-187
55
41
37
25
24
22
19
18
11
8
6
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
适用

646
313
68
20
14
10
10
6
4
3
2
2
1
1
1
1
宿主
小鼠
503
213
170
23
6
4
克隆类型
单克隆
标记
This CD40 antibody is un-conjugated
87
85
50
36
24
21
17
15
14
13
9
9
9
9
9
9
9
8
7
7
7
7
6
6
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Western Blotting (WB)
580
244
207
156
112
103
101
64
47
34
19
15
13
13
9
6
6
4
3
3
3
3
2
2
1
1
1
1
1
1
1
选项
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产品编号 ABIN968495
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.690664 ABIN968494 IF WB Mouse IgG1 AA 71-187 Log in to see 41-CD40 4
11.690664 ABIN1106353 EIA IF Mouse IgG1 Log in to see 9G10 3
11.690664 ABIN2704615 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
11.690664 ABIN1997455 FACS IF IF/ICC Mouse IgG1 AA 1-193 Log in to see 17
11.690664 ABIN1997449 FACS IF IF/ICC Mouse AA 1-193 Log in to see 03
11.690664 ABIN931290 IF ELISA Mouse IgG1 Log in to see 9G10
11.690664 ABIN4293032 ELISA ICC IF Mouse IgG1 Log in to see 9G10
11.690664 ABIN1326967 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 5G10
11.690664 ABIN1326962 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4F1
11.690664 ABIN1326971 IF ELISA Mouse IgG1 kappa AA 21-193, partial Log in to see 4G15
11.690664 ABIN1326964 IF ELISA WB Mouse IgG1 kappa AA 21-193, partial Log in to see 4F11
1 ABIN1112133 FACS IF PE Mouse IgG1 Log in to see HI40a 1
1 ABIN1112132 FACS IF FITC Mouse IgG1 Log in to see HI40a 1
1 ABIN1112130 FACS IF APC Mouse IgG1 Log in to see HI40a 1
1 ABIN2659262 FACS IF PE-Cy7 Mouse IgG1 kappa Log in to see 5C3 4
1 ABIN2665694 FACS IF IHC Mouse IgG1 kappa Log in to see HB14 8
1 ABIN467092 IF ELISA Mouse IgG1 Log in to see 9G10
1 ABIN1112134 FACS IF PerCP Mouse IgG1 Log in to see HI40a 1
1 ABIN2851695 FACS IF PE Mouse IgG2b Log in to see
1 ABIN2851737 FACS IF PE-Cy5 Mouse IgG2b Log in to see

General

抗原 CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) 抗体
抗原表位 AA 71-187
(55), (41), (37), (25), (24), (22), (19), (18), (11), (8), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(646), (313), (68), (20), (14), (10), (10), (6), (4), (3), (2), (2), (1), (1), (1), (1)
宿主 小鼠
(503), (213), (170), (23), (6), (4)
克隆类型 (克隆位点)
单克隆   ( )
标记 This CD40 antibody is un-conjugated
(87), (85), (50), (36), (24), (21), (17), (15), (14), (13), (9), (9), (9), (9), (9), (9), (9), (8), (7), (7), (7), (7), (6), (6), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunofluorescence (IF), Western Blotting (WB)
(580), (244), (207), (156), (112), (103), (101), (64), (47), (34), (19), (15), (13), (13), (9), (6), (6), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4个引用可参考
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CD40抗体详情

CD40目标详情 使用细节 贮存及处理 References for anti-CD40 antibody (ABIN968495) 图像
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Human CD40 aa. 71-187
克隆位点 41-CD40
亚型 IgG1

CD40目标详情

CD40抗体详情 使用细节 贮存及处理 References for anti-CD40 antibody (ABIN968495) 图像 回页首
抗原
别名 CD40 (CD40 Antibody 摘要)
背景 CD40, a member of the TNF receptor family, contains a cysteine-rich N-terminal domain and a Ser/Thr-rich region preceding the transmembrane domain. On B cells, signaling through CD40 induces cell growth and differentiation, mediates cell survival within the germinal center, and upregulates the expression of costimulatory and adhesion molecules, such as B7.1, B7.2, and ICAM-1. The interaction of CD40 on B cells and CD40L on activated CD4+ T cells is essential for immune functions, such as immuoglobulin class switching. Signal transduction through CD40 pathways involves interaction with proteins such as TRAFs (TRAF2, TRAF3, TRAF5, and TRAF6), Jak 3, and Tyr phosphorylation of proteins, such as Lyn, Syk, PI-3-kinase, STAT3, and STAT5. In TRAF2-deficient mice, CD40-mediated B cell proliferation and NFkappaB activation are defective. Ku70 and Ku80 associate with the membrane-proximal region of CD40 in human primary B cells and the engagement of CD40 leads translocation of Ku proteins to the nucleus. Thus, CD40 interacts with a variety of signal transducers which mediate its role in B cell survival, growth, differentiation, and immunoglobulin class switching.
分子量 44 kDa
研究领域 Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Inflammation, Cancer
途径 NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

使用细节

CD40抗体详情 CD40目标详情 贮存及处理 References for anti-CD40 antibody (ABIN968495) 图像 回页首
说明

Related Products: ABIN968584, ABIN967389

限制 仅限研究用

贮存及处理

CD40抗体详情 CD40目标详情 使用细节 References for anti-CD40 antibody (ABIN968495) 图像 回页首
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20°C.

References for anti-CD40 antibody (ABIN968495)

CD40抗体详情 CD40目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Morio, Hanissian, Bacharier, Teraoka, Nonoyama, Seki, Kondo, Nakano, Lee, Geha, Yata: "Ku in the cytoplasm associates with CD40 in human B cells and translocates into the nucleus following incubation with IL-4 and anti-CD40 mAb." in: Immunity, Vol. 11, Issue 3, pp. 339-48, 1999

Nguyen, Duncan, Mirtsos, Ng, Speiser, Shahinian, Marino, Mak, Ohashi, Yeh: "TRAF2 deficiency results in hyperactivity of certain TNFR1 signals and impairment of CD40-mediated responses." in: Immunity, Vol. 11, Issue 3, pp. 379-89, 1999

Randall, Heath, Santos-Argumedo, Howard, Weissman, Lund: "Arrest of B lymphocyte terminal differentiation by CD40 signaling: mechanism for lack of antibody-secreting cells in germinal centers." in: Immunity, Vol. 8, Issue 6, pp. 733-42, 1998

背景

Stamenkovic, Clark, Seed: "A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas." in: The EMBO journal, Vol. 8, Issue 5, pp. 1403-10, 1989

图像

CD40抗体详情 CD40目标详情 使用细节 贮存及处理 References for anti-CD40 antibody (ABIN968495) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) Western blot analysis of CD40 on EB1 cell lysate. Lane 1: 1:2500, lane 2: 1:5000, lan...
Immunofluorescence (IF) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) Immunofluorescent staining of HeLa cells.
 image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (Image 3)
Western Blotting (WB) image for anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (ABIN968495) anti-CD40 Molecule, TNF Receptor Superfamily Member 5 (CD40) (AA 71-187) antibody (Image 4)