anti-Broad Bean (Vicia faba) TUBB3 抗体 for Immunofluorescence

Recommended TUBB3 Antibody (supplied by: Log in to see )

抗原
Tubulin, Beta, 3 (TUBB3) 抗体
  • tubb3
  • TUBB3
  • TUBB4
  • CDCBM
  • CFEOM3A
  • beta-4
  • 3200002H15Rik
  • M(beta)3
  • M(beta)6
  • AI325297
  • M(beta)4
  • Tubb
  • Tubb4
  • tubulin, beta 3 class III
  • beta-tubulin 3
  • similar to tubulin, beta 3
  • tubulin, beta, 4
  • tubulin, beta 3
  • beta tubulin3
  • tubulin, beta 2C
  • tubulin, beta 4A class IVA
  • tubb3
  • TUBB3
  • tub3
  • TUBB2C
  • Tubb3
  • Tubb4a
抗原表位
C-Term
47
44
20
15
13
6
5
4
4
4
4
3
3
2
2
1
1
1
1
1
适用
Broad Bean (Vicia faba)
240
117
104
23
20
15
15
8
8
6
6
5
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
宿主
小鼠
127
123
19
14
1
克隆类型
单克隆
标记
This TUBB3 antibody is un-conjugated
24
13
12
5
5
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
239
107
78
64
64
55
47
19
13
13
11
8
8
5
2
2
2
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产品编号 ABIN110980
4,544.82 ¥
(含税, 含运费)
来自4A Biotech Co.,Ltd.(本地经销商)
获取报价
中国 北京 101111
No. 88 KeChuang 6th Street
Room 801-803
Beijing Economic Technological Development Area
4A Biotech Co.,Ltd.
Tel 400-7060-959
传真 +86 10 56315212-8813
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN110982 EIA FACS IHC (fro) IF IHC (p) WB Mouse IgG1 N-Term Log in to see TU-20 10
1 ABIN110981 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG1 N-Term Log in to see TU-20 10

引用最多的anti-TUBB3 抗体 for Immunofluorescence

Similar anti-TUBB3 抗体

应用范围 / 适用 Broad Bean (Vicia faba)
Enzyme Immunoassay (EIA) 3 抗体
Flow Cytometry (FACS) 3 抗体
Immunofluorescence (IF) 3 抗体
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 抗体
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 抗体
Western Blotting (WB) 4 抗体

General

抗原 Tubulin, Beta, 3 (TUBB3) 抗体
抗原表位 C-Term
(47), (44), (20), (15), (13), (6), (5), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
适用 Broad Bean (Vicia faba)
(240), (117), (104), (23), (20), (15), (15), (8), (8), (6), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(127), (123), (19), (14), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This TUBB3 antibody is un-conjugated
(24), (13), (12), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(239), (107), (78), (64), (64), (55), (47), (19), (13), (13), (11), (8), (8), (5), (2), (2), (2)
Pubmed 6个引用可参考
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TUBB3抗体详情

TUBB3目标详情 使用细节 贮存及处理 ProductDetails: References for anti-TUBB3 antibody (ABIN110980) 图像
特异性 This antibody recognizes the C-terminal peptide sequence ESESQGPK (aa 441-448) of human class III β-tubulin specific for neurones. The antibody is a highly specific marker for neuronal tissue. TU-20 is very useful for the detection of microtubule structures on fixed cells. MAb TU-20 is widely cross-reactive among species (recognized epitope conserved within all species).
交叉反应 (详细) Species reactivity (tested):Human and Rat.
纯化方法 Purified
免疫原 Synthetic C-terminal peptide
克隆位点 TU-20
亚型 IgG1

TUBB3目标详情

TUBB3抗体详情 使用细节 贮存及处理 ProductDetails: References for anti-TUBB3 antibody (ABIN110980) 图像 回页首
抗原
别名 TUBB3 / TUBB4 (TUBB3 Antibody 摘要)
背景 Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10 % of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy terminal. For beta tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2.Synonyms: Tubulin beta-3 chain, Tubulin beta-4, Tubulin beta-III
基因ID 10381
NCBI登录号 NP_001184110
UniProt Q13509
途径 Microtubule Dynamics, M Phase

使用细节

TUBB3抗体详情 TUBB3目标详情 贮存及处理 ProductDetails: References for anti-TUBB3 antibody (ABIN110980) 图像 回页首
应用备注 Immunohistochemistry on Frozen Sections: 5-10 μg/mL (1/40-1/80). Immunohistochemistry on Paraffin Sections: 20 μg/mL (1/20). Proteinase K treatment forantigen retrieval is recommended. Recommended Positive Control: Human cortex. Has been described to work in ELISA and Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer’s hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS6. Block with 10% normal goat serum in PBS for 30min. in a humid chamber7. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber8. Wash in PBS9. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber10. Wash in PBS11. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 12. Wash in PBS13. Counterstain with Mayer’s hemalum
限制 仅限研究用

贮存及处理

TUBB3抗体详情 TUBB3目标详情 使用细节 ProductDetails: References for anti-TUBB3 antibody (ABIN110980) 图像 回页首
溶解方式 Restore in 0.5 mL distilled water.
浓度 0.4 mg/mL
缓冲液 Stock solution contains PBS pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

ProductDetails: References for anti-TUBB3 antibody (ABIN110980)

TUBB3抗体详情 TUBB3目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998