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BlueAQUA Prestained Protein Ladder

ABIN2868520 产品详细信息, 供应商: Log in to see
检测范围
10-180 kDa
最低检测浓度
10 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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原理 The BlueAQUA Prestained Protein Ladder is a blue protein standard with 11 pre-stained proteins covering a wide range molecular weights from 10 to180 kDa.
检测方法 Colorimetric
产品特性 Proteins are covalently coupled with a blue chromophore, and two reference bands (at 25 kDa and 72 kDa respectively) are enhanced in intensity when separated on SDS-PAGE (Tris-glycine buffer).The BlueAQUA Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
应用备注 - 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 1.5~2.5 μl per well for general Western transferring.
- Apply more for thicker (> 1.5 mm) or larger gel.
试剂准备

Quality Control: Under suggested conditions, BlueAQUA Prestained Protein Ladder resolves 11 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.

限制 仅限研究用
状态 Liquid
缓冲液 Approximately 0.1~0.5 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
储存条件 -20 °C
储存方法 Stable for up to 2 weeks at 25°C Stable for up to 3 months at 4°C For long term storage, store at -20°C
厂商提供的图像
SDS-PAGE (SDS) image for BlueAQUA Prestained Protein Ladder (ABIN2868520) BlueAQUA Prestained Protein Ladder