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BLUltra Prestained Protein Ladder

ABIN2868518 产品详细信息, 供应商: Log in to see
检测范围
6.5-270 kDa
最低检测浓度
6.5 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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原理 The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa.
检测方法 Colorimetric
产品特性 Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
应用备注 - 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
- 2.5 μl per well for general Western transferring.
试剂准备

Quality Control: Under suggested conditions, BLUltra Prestained Protein Ladder resolves 10 major bands in 4-12 % Bis-Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.

限制 仅限研究用
状态 Liquid
缓冲液 Storage Buffer: 62.5 mM Tris-H3PO4 ( pH 7.5 at 25 °C), 1 mM EDTA, 2 % SDS, 10 mM DTT, 1 mM Sodium azide, 33 % glycerol.
储存条件 -20 °C
储存方法 Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C.For long term storage, store at -20°C.
厂商提供的图像
SDS-PAGE (SDS) image for BLUltra Prestained Protein Ladder (ABIN2868518) BLUltra Prestained Protein Ladder
SDS-PAGE (SDS) image for BLUltra Prestained Protein Ladder (ABIN2868518) BLUltra Prestained Protein Ladder (Image 2)