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OneMARK B DNA Ladder

250-10000 bp
产品编号 ABIN2868516
发货至: 中国
  • 检测范围
    250-10000 bp
    最低检测浓度
    250 bp
    应用范围
    Agarose Gel Electrophoresis (AGE)
    原理
    OneMARK B (Ready-to-Use)
    产品特性
    OneMARK B with the Novel Green was designed to show virtually uniform spacing over a wide fragment range. The ladder is supplied in a ready-to-use format containing the fluorescent DNA stain and tracking dyes. High quantum yield and excellent stability make the fluorescence dye the ideal fluorophore for DNA staining applications and a superior replacement for the widely used dyes, ethidium bromide or SYBR® Green I.The OneMARK B with the Novel Green was optimized for direct loading onto unstained agarose gels. The ladders provide the highest level of convenience during the routine handling and avoid commonly used gel staining procedures with the ethidium bromide or SYBR® Green I.The OneMARK B includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg per loading) for approximating the mass of DNA in comparably intense samples of similar size.
  • 应用备注
    No-post-staining procession, Direct loading onto your agarose gel for analysis
    说明

    Range: 250-10,000 bp
    Number of bands: 13
    Recommended Load: 6 μl / well,Containing bromophenol blue and xylene cyanol FF as the tracking dyes.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    PCR products and double-stranded DNA digested with appropriate restriction enzymes are phenol-extracted and equilibrated to 10 mM Tris-HCl ( pH 8.0) and 1 mM EDTA.
    注意事项
    OneMARK B is light sensitive and should be stored and protected from light.
    储存条件
    -20 °C
    储存方法
    Store at RT and 4°C up to 6 months. Store at -20°C up to 1 year.
    有效期
    12 months
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