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The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Recommendations for Loading 1.Thaw the ladder either at room temperature or at 37-40 °C for a few minutes to dissolve precipitated solids. Do not boil. 2.Mix thoroughly to ensure the solution is homogeneous.3.Load the following volumes of the ladder on SDS-polyacrylamide gel:- 5 μL per well for mini-gels, 2.5 μL per well for blots- 10 μL per well for large gels, 5 μL per well for blots