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PiNK Plus Prestained Protein Ladder

ABIN2868256 产品详细信息, 供应商: Log in to see
检测范围
10-175 kDa
最低检测浓度
10 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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原理 The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa.
检测方法 Colorimetric
产品特性 Broad range: 10-175 kDa. Easy to identify: includes the -10, -40 and 90 kDa reference bands coupled with an blue dye.
应用备注 Can be used for monitoring of protein migration during SDS-polyacrylamide gel electrophoresis and monitoring of protein transfer onto membranes during Western blotting.
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
说明

The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

实验流程

Recommendations for Loading
1.Thaw the ladder either at room temperature or at 37-40 °C for a few minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly to ensure the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5 μL per well for mini-gels, 2.5 μL per well for blots
- 10 μL per well for large gels, 5 μL per well for blots

限制 仅限研究用
状态 Liquid
缓冲液 Approximately 0.2~0.4 mg/mL of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25 °C), 2 % SDS, 1 mM 2-Mercaptoethanol, 3.6 M Urea, and 15 % (v/v) glycerol).
储存条件 RT
储存方法 Store at 25°C for 6 months, Store at -20°C for 24 months
有效期 24 months
厂商提供的图像
 image for PiNK Plus Prestained Protein Ladder (ABIN2868256) PiNK Plus Prestained Protein Ladder