BlueRAY Prestained Protein Ladder
-
- 检测方法
- Colorimetric
- 检测范围
- 10-180 kDa
- 最低检测浓度
- 10 kDa
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB)
-
BLUltra Prestained Protein Ladder
6.5-270 kDa Colorimetric
Protein Molecular Weight Marker14-120 kDa Colorimetric
100bp DNA Ladder100-1500 bp
OneMARK B DNA Ladder250-10000 bp
OneMARK 100 DNA Ladder100-3000 bp
50bp DNA Ladder50-1,500 bp Fluorometric
BlueAQUA Prestained Protein Ladder10-180 kDa Colorimetric
BLUeye Prestained Protein Ladder10-245 kDa Colorimetric
BLUelf Prestained Protein Ladder5 kDa - 245 kDa Colorimetric
ExcelBand™ 3-color Regular Range Protein Marker10 kDa - 180 kDa
-
- 应用备注
-
Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2-3 μL per well for general Western transfering. Apply more for thicker (>1.5mm) or larger gel.
5 μL of BlueRAY Prestained Protein Ladder resolves 10 bands in 4-20 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane. - 说明
-
The BlueRAY Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights for 10 to 180 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BlueRAY Prestained Protein Ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. The ladder is supplied in gel loading buffer and is ready to use.Do not heat, dilute, add reducing agent before loading.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Tris-Glycine Bis-Tris 12 % Bis-Tris 10 % 4~20 % MOPS buffer MES buffer
- 储存条件
- RT
- 储存方法
- Store at 25°C for 6 months, Store at -20°C for 24 months
- 有效期
- 24 months
-
