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BLUeye Prestained Protein Ladder

ABIN2868252 产品详细信息, 供应商: Log in to see
检测范围
10-245 kDa
最低检测浓度
10 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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检测方法 Colorimetric
应用备注 Use 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 1.5-2.5 μL per well for general Western transfering.
BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Tris-glycine buffer) and after Western blotting to nitrocellulose membrane.
说明

The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

试剂准备

Quality Control:
Under suggested conditions, BLUeye Prestained Protein Ladder resolves 12 major bands in 15 % SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.

限制 仅限研究用
状态 Liquid
缓冲液 Approximately 0.1~0.4 mg/mL of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25 °C), 2 % SDS, 1 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). Bis-Tris 10 % Tris-Glycine Bis-Tris 10 % MES buffer 4~20 % MOPS buffer
储存条件 RT
储存方法 Store at 25°C for 6 months, Store at -20°C for 24 months
有效期 24 months
厂商提供的图像
 image for BLUeye Prestained Protein Ladder (ABIN2868252) BLUeye Prestained Protein Ladder