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Lambda DNA/Hind III Marker

ABIN1540476 产品详细信息, 供应商: Log in to see
检测范围
564-23130 bp
最低检测浓度
564 bp
应用范围
Agarose Gel Electrophoresis (AGE)
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原理 Lambda DNA/ Hind III Marker is valuable for various DNA sizing purposes.
检测方法 Fluorometric
产品特性 Lambda DNA/Hind III Marker was digested with restriction enzyme Hind III, followed by phenol/chloroform extraction to inactivate the enzyme and dialyzed in TE buffer. The final DNA concentration is measured at OD260 and compared to other DNA standards for accuracy. This ladder is pre-mixed with loading dye and is ready to use.
7 DNA Fragments (bp): 564, 2027, 2322, 4361, 6557, 9416, and 23130

Range: 564bp-23130bp
Bands: 7
应用备注 Recommended Loading: 6µL/Lane.
Add 6µL ladder directly to each well. You may need more or less than 6µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 6µL/Lane, 8cm, 0.8% Agarose Gel, 0.5xTAE, 7V/cm, 45min.
说明

Do not heat before loading.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

限制 仅限研究用
状态 Liquid
储存条件 -20 °C
储存方法 3 months at room temperature, for long term storage, please keep at -20°C.
有效期 3 months
厂商提供的图像
Agarose Gel Electrophoresis (AGE) image for Lambda DNA/Hind III Marker (ABIN1540476) Lambda DNA/Hind III Marker