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20bp DNA Ladder

ABIN1540464 产品详细信息, 供应商: Log in to see
检测范围
60-300 bp
最低检测浓度
60 bp
应用范围
Agarose Gel Electrophoresis (AGE)
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原理 20bp Ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs.
检测方法 Fluorometric
产品特性 The ladder consists of 13 linear double-stranded fragments. The 100bp and 200bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 200bp are 40ng. The 100bp and 200bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.
13 DNA Fragments (bp): 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, and 300
100bp and 200bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 60bp-300bp
Bands: 13
应用备注 Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 4% Agarose Gel, 1×TBE, 5V/cm, 1h.
说明

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

限制 仅限研究用
状态 Liquid
储存条件 -20 °C
储存方法 1 year at room temperature, for long term storage, please keep at -20°C.
有效期 12 months
厂商提供的图像
Agarose Gel Electrophoresis (AGE) image for 20bp DNA Ladder (ABIN1540464) 20bp DNA Ladder