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LMW DNA Ladder

25-700 bp Fluorometric
产品编号 ABIN1540462
发货至: 中国
  • 检测方法
    Fluorometric
    检测范围
    25-700 bp
    最低检测浓度
    25 bp
    应用范围
    Agarose Gel Electrophoresis (AGE)
    原理
    LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs.
    产品特性
    The ladder consists of 10 linear double-stranded DNA fragments, chromatography- purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
    10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700
    100bp and 300bp Bands: 100ng/5 µL
    Other Bands: 40ng/5 µL
    Range: 25bp-700bp
    Bands: 10
  • 应用备注
    Recommended Loading: 5 µL/Lane.
    Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
    Recommended Loading: 5 µL/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.
    说明

    Do not heat before loading.
    For quantification, adjust the concentration of the sample to equalize.
    Visualize DNA by staining with ethidium bromide or with SYBR Green I.

    限制
    仅限研究用
  • 状态
    Liquid
    储存条件
    -20 °C
    储存方法
    1 year at room temperature, for long term storage, please keep at -20°C.
    有效期
    12 months
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