NEW: 请通过本地经销商订购 4A Biotech Co.,Ltd.

LMW DNA Ladder

ABIN1540462 产品详细信息, 供应商: Log in to see
检测范围
25-700 bp
最低检测浓度
25 bp
应用范围
Agarose Gel Electrophoresis (AGE)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

原理 LMW DNA Ladder is ideal for determining the size of double-stranded DNA from 25 to 700 base pairs.
检测方法 Fluorometric
产品特性 The ladder consists of 10 linear double-stranded DNA fragments, chromatography- purified. The intensity of the 100bp and 300bp has been increased to serve as a reference for easy identification. All fragments are precisely quantified and mixed during the production. For 5 µL loading, all fragments except 100bp and 300bp are at 40ng. The 100bp and 300bp fragments are at 100ng. This ladder is pre-mixed with loading dye and is ready to use.
10 DNA Fragments (bp): 25, 50, 75, 100, 150, 200, 300, 400, 500, and 700
100bp and 300bp Bands: 100ng/5 µL
Other Bands: 40ng/5 µL
Range: 25bp-700bp
Bands: 10
应用备注 Recommended Loading: 5 µL/Lane.
Add 5 µL ladder directly to each well. You may need more or less than 5 µL of ladder, depending on well size and level of intensity needed to visualize the bands.
Recommended Loading: 5 µL/Lane, 8cm, 3 % Agarose Gel, 0.5×TBE, 5V/cm, 1h.
说明

Do not heat before loading.
For quantification, adjust the concentration of the sample to equalize.
Visualize DNA by staining with ethidium bromide or with SYBR Green I.

限制 仅限研究用
状态 Liquid
储存条件 -20 °C
储存方法 1 year at room temperature, for long term storage, please keep at -20°C.
有效期 12 months
厂商提供的图像
Agarose Gel Electrophoresis (AGE) image for LMW DNA Ladder (ABIN1540462) LMW DNA Ladder