Prestained Dual Color Protein Molecular Weight Marker

ABIN1113047 产品详细信息, 供应商: Log in to see
检测范围
10-250 kDa
最低检测浓度
10 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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原理 Prestained Protein Molecular Weight Marker is a three-color molecular weight standard, contains 9 prestained natural proteins with molecular weights from 10 kDa to 250 kDa. It has two orange reference bands-70 kDa band and 25 kDa band.
检测方法 Colorimetric
产品特性 Prestained protein ladders / marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins.
板类型 Strips (12 x 8)
实验流程
  1. Thaw the ladder either at room temperature or at 37°C for a few minutes to dissolve precipitated solids thoroughly. Do not boil! 2. Mix gently, but thoroughly, to ensure that the solution is homogeneous. 3. Load the following volumes of the ladder on Min SDS-polyacrylamide gel: 5 myl per well, with a thickness of 0.75-1.0 mm Large SDS-polyacrylamide gel: 10 myl per well, with a thickness of 0.75-1.0 mm
限制 仅限研究用
状态 Liquid
缓冲液 62.5 mM Tris-H3PO4 (pH 7.5, 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
储存液 Sodium azide, Thimerosal (Merthiolate)
储存条件 4 °C/-20 °C
储存方法 Store at 4 °C for 3 months, and at -20 °C for long term.