SIX2 293 Cell Lysate

ABIN1600776 产品详细信息, 供应商: Log in to see
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  • six1b
  • six2
  • zgc:92332
  • six2-A
  • sine oculis homeobox homolog 1a
  • sine oculis-related homeobox 2
  • SIX homeobox 2
  • six1a
  • Six2
  • six2
  • SIX2
宿主

资源
HEK293T
标记
Myc-DYKDDDDK Tag
应用范围
Western Blotting (WB)
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产品特性 Antigen standard for SIX homeobox 2 (SIX2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid (True ORF cDNA clone RC201328, OriGene Technologies, Inc.) and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
组件 This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer.
别名 SIX2
背景 Synonyms: sine oculis homeobox homolog 2, Homeobox protein SIX2
NCBI登录号 NP_058628
研究领域 Organogenesis, Transcription Factors
应用备注 WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 µg lysate per lane.
限制 仅限研究用
状态 Liquid
缓冲液 Each lysate vial contains 0.1 mg lysate in 0.1 mL (1 mg/mL) of RIPA Buffer (50 mM Tris-HCl pH 7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1 % NP40). The loading buffer vial contains 0.5 mL 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10 % glycerol, 4 % SDS, 0.002 % Bromophenol blue, 5 % beta-mercaptoethanol).
注意事项 Minimize freeze-thaw cycles.
储存条件 -80 °C
储存方法 Store at -80 °C. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20 °C.
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for SIX2 293 Cell Lysate (ABIN1600776) SIX2 lysate validation: The SIX2 293 Cell Lysate is used in Western blot to detect ta...