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Competitive ELISA

Competitive ELISAs for protein detection in a wide variety of sample types. In a competitive format assay, as antigen concentration in a sample increases, signal intensity decreases.The central concept behind a competitive ELISA is that a larger quantity of analyte in a sample results in fewer free antibodies in solution and by extension a smaller number of labeled antibodies bound to the standard on the plate.

A primary antibody (unlabeled) is incubated with sample antigen. They are then added to 96-well plates which are pre-coated with the same antigen, followed by a washing step. Antigen quantity in the sample determines the amount antibody binding on the well. The secondary antibody that is specific to the primary antibody and conjugated with an enzyme is added. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The competitive assay is an appealing option if no suitable antibody pair can be identified for sandwich ELISA, or if the analyte in question is too small to permit binding of both a capture and detection antibody. Our support team will gladly help you with finding the right ELISA kit for your needs. Please contact us via phone, email or use our live chat system.

antibodies-online.com offers competitive ELISA kits for thousands of targets and for a large number of species and sample types. Use our search below or contact us to find the product you need.

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25,575 results :

适用: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed 24 references Independent Validation 1 validation
产品编号 ABIN6574100
适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed 43 references
产品编号 ABIN6574085
适用: 兔 Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed 2 references
产品编号 ABIN6574090
CORT 适用: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Plasma, Serum, Urine
Pubmed 25 references
产品编号 ABIN6574088
AMH 适用: 人 Colorimetric Competition ELISA 937.5 pg/mL - 15000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed 18 references
产品编号 ABIN6574075
ADM 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pubmed 5 references
产品编号 ABIN6574074
LH 适用: 大鼠 Colorimetric Competition ELISA 98.77 pg/mL - 8000 pg/mL Plasma, Serum
Pubmed 28 references
产品编号 ABIN6574078
MT 适用: Various Species Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Pubmed 23 references
产品编号 ABIN6574097
TSH 适用: 小鼠 Colorimetric Competition ELISA 6.4 pg/mL - 4000 pg/mL Plasma, Serum
Pubmed 21 references Independent Validation 1 validation
产品编号 ABIN415519
适用: 其他 Colorimetric Competition ELISA Cell Samples, Plasma, Serum, Tissue Samples, Urine
Pubmed 144 references
产品编号 ABIN2344960
  • 类型 Kit
    • Kit
  • 抗原
    • Testosterone
    • Cortisol
    • Thyroxine T4 (T4)
    • Luteinizing Hormone (LH)
    • Albumin (ALB)
    • E2 Estradiol
    • FSH
    • Progesterone
    • Triiodothyronine T3 (T3)
    • HbA1c
    • ACTH
    • APOB
    • Vitamin A
    • Serotonin (5-HT)
    • CRH
    • Prolactin (PRL)
    • beta Endorphin (beta-EP)
    • NPPC
    • TSH
    • AMBP
    • HVD3
    • Growth Hormone 1 (GH1)
    • Prostaglandin F2alpha
    • Thromboxane B2 (TXB2)
    • Acetyl-CoA Carboxylase
    • APOE
    • Dopamine (DA)
    • Free Triiodothyronine T3 (fT3)
    • IgM
    • Insulin (INS)
    • Malondialdehyde (MDA)
    • Pentosidine
    • Relaxin
    • Somatostatin (SST)
    • VWF
    • Aldosterone (ALD)
    • Dihydrotestosterone (DHT)
    • Leptin (LEP)
    • Melatonin (MT)
    • 1,25 Dihydroxy Vitamin D3 (DHVD3)
    • HNE
    • Adrenomedullin (ADM)
    • Carboxy Methyl Lysine (CML)
    • Clusterin (CLU)
    • DHEA
    • GLP-1
    • IgG
    • KNG1
    • SAA
    • SOD2
    Please use search to find more...
  • 应用范围 ELISA
    • ELISA
    • Colloidal Gold Immunochromatography Assay (GICA)
    • Endpoint Fluorescent Competitive Immunoassay (EFCIA)
    • Multiplex ELISA (mpELISA)
  • 适用
    • Human
    • Mouse
    • Rat
    • Rabbit
    • Guinea Pig
    • Dog
    • Monkey
    • Sheep
    • Cow
    • Goat
    • Chicken
    • Pig
    • Various Species
    • Chemical
    • Horse
    • Fish
    • Hormone
    • Mammalian
    • Others
    • Rhesus Monkey
    • Duck
    • Plant
    • Borrelia burgdorferi
    • Hamster
    • Peanut
    • Bacteria
    • Epstein-Barr Virus (EBV)
    • Grouper (Epinephelinae)
    • Measles Virus
    • Mumps Virus
    • Orientia tsutsugamushi
    • Salmon
    • Varicella Zoster Virus (VZV)
    • Arabidopsis
    • Camel
    • Chlamydia trachomatis
    • Corynebacterium diphtheriae
    • Cyanobacteria (Nostoc)
    • Deer
    • Fungus
    • Listeria monocytogenes
    • Oat
    • Penicillium sp.
    • Red pepper
    • Rodents
    • Soybean
    • Streptomyces
    • Tag
    • Tomato
    • Toxoplasma gondii
    Please use search to find more...
  • 论文引用
  • 图像
  • 独立验证
  • 实验类型 Competition ELISA
    • Competition ELISA
    • Sandwich ELISA
    • Cell ELISA
    • DNA-Binding ELISA
    • Indirect ELISA
    • Bridging ELISA
  • Analytical Method
    • Quantitative
    • Qualitative
    • Qualitative and Quantitative
    • Semi-Quantitative
    • Qualitative and Semi-Quantitative
  • 检测方法
    • Colorimetric
    • Fluorometric, Colorimetric
  • Plate
    • Pre-coated
    • Strips (12 x 8)
    • Pre-coated,Strips (12 x 8)
    • Uncoated
    • Strips
  • 抗原表位
    • phosphorylated
    • Soluble
    • Nuclear
    • phosphospecific
    • Whole Molecule
    • free
    • C-Term
    • N-Term
    • Arg1
    • total
    • Precursor
    • Regulatory Subunit 1
    • Regulatory Subunit 10
    • Light Chain
    • Regulatory Subunit 12
    • Regulatory Subunit 13
    • Regulatory Subunit 2
    • Chain kappa
    • Chain lambda
    • Fc Region
    • N-Term Kinases/stress-activated Protein Kinase
    • pSer880
    • pThr180
    • pTyr182
  • 标记
    • 非结合性
  • 样本
    • Serum
    • Plasma
    • Tissue Homogenate
    • Cell Culture Supernatant
    • Cell Lysate
    • Urine
    • Saliva
    • Tissue Samples
    • Biological Agents
    • Milk
    • Cerebrospinal Fluid
    • Red Blood Cell Lysate
    • Cell Samples
    • Biological Fluids
    • Erythrocyte Lysates
    • Feed
    • Plant Tissue
    • Cell Culture Lysate
    • Food
    • Beverages
    • Cereals
    • Grains
    • Red Blood Cells
    • Vinegar
    • Cell Culture Cells
    • Edible oil
    • Egg white
    • Liver
    • Cell Culture Samples
    • Cell-free Extracts
    • Egg
    • Fecal
    • Juice
    • Muscle
    • Plasma (EDTA)
    • Plasma (citrate)
    • Plasma (heparin)
    • Platelet-Poor Plasma
    • Tissue Culture Medium
    • Tissue Lysate
    • Vegetables
    • Water
  • Grade
    • Small Sample
    • High Sensitivity
    • Wide Range
    • Instant
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