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CXCL12 ELISA 试剂盒

CXCL12 适用: 小鼠 Colorimetric Sandwich ELISA 3-600 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625176
发货至: 中国
  • 抗原 See all CXCL12 ELISA试剂盒
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    3-600 pg/mL
    最低检测浓度
    3 pg/mL
    应用范围
    ELISA
    原理
    Mouse SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    交叉反应 (详细)
    This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1beta, IL-2, IL-3, IL-3 Rb, IL- 4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M- CSF, MIG, MIP-1alpha, MIP-1gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF).
    灵敏度
    < 3 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our top product CXCL12 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    试剂准备
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants. Suggested dilution for normal serum/plasma: 2 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C. Add 600 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium) into Item C vial to prepare 9 ng/mL standard solution. Dissolve the powder thoroughly by a gentle mix. Add 40 µL SDF-1alpha standard from the vial of Item C, into a tube with 560 µL Assay Diluent A or 1x Assay Diluent B to prepare a 600 pg/mL stock standard solution. Pipette 300 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 40 µL standard + 560 µL 300 µL 300myl 300 µL 300 µL 300 µL 300 µL 600 300 150 75 37.5 18.75 9.38 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 80-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 200-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 50 µL of HRP-Streptavidin concentrate into a tube with 10 ml 1x Assay Diluent B to prepare a 200-fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    实验流程
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    结果分析

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Mouse SDF-1-alpha concentration (pg/mL) 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Mouse SDF-1-alpha concentration (pg/mL) 1 10 100 1000 O D =4 50 n m 0.01 0.1 1 10
    Sensitivity: The minimum detectable dose of SDF-1alpha is typically less than 3 pg/mL.
    Recovery: Recovery was determined by spiking various levels of mouse SDF-1alpha into mouse serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 98.76 88-106 Plasma 100.36 90-107 Cell culture media 99.34 89-107
    Linearity: Sample Type Serum Plasma Cell Culture Media 1:2 Average % of Expected 99 99 98 Range ( %) 91-107 89-106 88-106 1:4 Average % of Expected 97 98 96 Range ( %) 91-107 89-106 91-108
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    实验精密度
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    限制
    仅限研究用
  • 注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Zhang, Zhang, Liu, Zhang, Zhang, Tang: "Repairing sciatic nerve injury with an EPO-loaded nerve conduit and sandwiched-in strategy of transplanting mesenchymal stem cells." in: Biomaterials, Vol. 142, pp. 90-100, (2018) (PubMed).

    Tebebi, Burks, Kim, Williams, Nguyen, Venkatesh, Frenkel, Frank et al.: "Cyclooxygenase-2 or tumor necrosis factor-? inhibitors attenuate the mechanotransductive effects of pulsed focused ultrasound to suppress mesenchymal stromal cell homing to healthy and dystrophic ..." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 4, pp. 1173-86, (2015) (PubMed).

    Burks, Nguyen, Tebebi, Kim, Bresler, Ziadloo, Street, Yuen, Star, Frank: "Pulsed focused ultrasound pretreatment improves mesenchymal stromal cell efficacy in preventing and rescuing established acute kidney injury in mice." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 4, pp. 1241-53, (2015) (PubMed).

    Fulzele, Krause, Panaroni, Saini, Barry, Liu, Lotinun, Baron, Bonewald, Feng, Chen, Weinstein, Wu, Kronenberg, Scadden, Divieti Pajevic: "Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling." in: Blood, Vol. 121, Issue 6, pp. 930-9, (2013) (PubMed).

    Mallela, Ravi, Jean Louis, Mulaney, Cheung, Sree Garapati, Chinnasamy, Wang, Nagaraj, Mohapatra, Mohapatra: "Natriuretic peptide receptor A signaling regulates stem cell recruitment and angiogenesis: a model to study linkage between inflammation and tumorigenesis." in: Stem cells (Dayton, Ohio), Vol. 31, Issue 7, pp. 1321-9, (2013) (PubMed).

    Said, Frierson, Sanchez-Carbayo, Brekken, Theodorescu: "Loss of SPARC in bladder cancer enhances carcinogenesis and progression." in: The Journal of clinical investigation, Vol. 123, Issue 2, pp. 751-66, (2013) (PubMed).

    Wang, Yang, Qian, Zhang, Gao, Li, Wang, Wang: "Statin administration does not improve the mobilization of very small embryonic-like stem cells (VSELs) in contrast to resveratrol treatment in a murine model of acute myocardial infarction." in: Physiological research, Vol. 61, Issue 5, pp. 543-9, (2013) (PubMed).

    Pramanik, Sheng, Ichihara, Heisterkamp, Mittelman: "Adipose tissue attracts and protects acute lymphoblastic leukemia cells from chemotherapy." in: Leukemia research, Vol. 37, Issue 5, pp. 503-9, (2013) (PubMed).

    Lü, Hu, Chen, Peng, Chen, Hu, Teng, Liang: "miR-27b represses migration of mouse MSCs to burned margins and prolongs wound repair through silencing SDF-1a." in: PLoS ONE, Vol. 8, Issue 7, pp. e68972, (2013) (PubMed).

    Hamers, Vos, Rassam, Marinkovi?, Marincovic, Kurakula, van Gorp, de Winther, Gijbels, de Waard, de Vries: "Bone marrow-specific deficiency of nuclear receptor Nur77 enhances atherosclerosis." in: Circulation research, Vol. 110, Issue 3, pp. 428-38, (2012) (PubMed).

    Huang, Shih, Tsao, Lin, Huang, Wu, Lee, Kao, Chang, Nakagami, Morishita, Ou, Hou, Lin, Shyu, Lin: "Dipeptidyl peptidase-4 inhibitor improves neovascularization by increasing circulating endothelial progenitor cells." in: British journal of pharmacology, Vol. 167, Issue 7, pp. 1506-19, (2012) (PubMed).

    Vagima, Levy, Gur, Tidhar, Aftalion, Abramovich, Zahavy, Zauberman, Flashner, Shafferman, Mamroud: "Early sensing of Yersinia pestis airway infection by bone marrow cells." in: Frontiers in cellular and infection microbiology, Vol. 2, pp. 143, (2012) (PubMed).

    Lü, Li, Hu, Fan, Wang, Wu, Liang, Yang: "microRNA-27b suppresses mouse MSC migration to the liver by targeting SDF-1αin vitro." in: Biochemical and biophysical research communications, Vol. 421, Issue 2, pp. 389-95, (2012) (PubMed).

    Li, Chang, Lathia, Wang, Pacenta, Cotleur, Ransohoff: "Chemokine receptor CXCR4 signaling modulates the growth factor-induced cell cycle of self-renewing and multipotent neural progenitor cells." in: Glia, Vol. 59, Issue 1, pp. 108-18, (2011) (PubMed).

    Fischer, Din, Gude, Konstandin, Wu, Quijada, Sussman: "Cardiac progenitor cell commitment is inhibited by nuclear Akt expression." in: Circulation research, Vol. 108, Issue 8, pp. 960-70, (2011) (PubMed).

    Bleul, Fuhlbrigge, Casasnovas, Aiuti, Springer: "A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1)" in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1101-9, (1997) (PubMed).

    Shirozu, Nakano, Inazawa, Tashiro, Tada, Shinohara, Honjo: "Structure and chromosomal localization of the human stromal cell-derived factor 1 (SDF1) gene." in: Genomics, Vol. 28, Issue 3, pp. 495-500, (1996) (PubMed).

    Gale, Butturini: "Emergency response to nuclear and radiation accidents." in: Bone marrow transplantation, Vol. 4 Suppl 4, pp. 2-3, (1990) (PubMed).

  • 抗原 See all CXCL12 ELISA试剂盒
    CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
    别名
    SDF-1 alpha (CXCL12 产品)
    别名
    IRH ELISA Kit, PBSF ELISA Kit, SCYB12 ELISA Kit, SDF1 ELISA Kit, TLSF ELISA Kit, TPAR1 ELISA Kit, CINC-1 ELISA Kit, Gro1 ELISA Kit, Pbsf ELISA Kit, Scyb12 ELISA Kit, Sdf1 ELISA Kit, Tlsf ELISA Kit, Tpar1 ELISA Kit, Fsp ELISA Kit, KC ELISA Kit, Mgsa ELISA Kit, N51 ELISA Kit, Scyb1 ELISA Kit, gro ELISA Kit, sdf-1 ELISA Kit, sdf1 ELISA Kit, xSDF-1 ELISA Kit, SDF-1 ELISA Kit, cxcl12 ELISA Kit, sdf-1a ELISA Kit, sdf1a ELISA Kit, wu:fa55e10 ELISA Kit, wu:fc16h12 ELISA Kit, wu:fj84c02 ELISA Kit, C-X-C motif chemokine ligand 12 ELISA Kit, C-X-C motif chemokine ligand 1 ELISA Kit, chemokine (C-X-C motif) ligand 12 ELISA Kit, chemokine (C-X-C motif) ligand 1 ELISA Kit, C-X-C motif chemokine ligand 12 L homeolog ELISA Kit, chemokine (C-X-C motif) ligand 12a (stromal cell-derived factor 1) ELISA Kit, CXCL12 ELISA Kit, cxcl12 ELISA Kit, Cxcl1 ELISA Kit, Cxcl12 ELISA Kit, cxcl12.L ELISA Kit, cxcl12a ELISA Kit
    背景
    Stromal cell-derived factor 1 (SDF-1) (12-O-tetradecanoylphorbol 13-acetate repressed protein 1) (TPAR1) (C-X-C motif chemokine 12) (Pre-B cell growth-stimulating factor) (PBSF) (Thymic lymphoma cell-stimulating factor) (TLSF)
    基因ID
    20315
    UniProt
    P40224
    途径
    Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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