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CXCL9 ELISA 试剂盒

CXCL9 适用: 人 Colorimetric Sandwich ELISA 20-6000 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625045
发货至: 中国
  • 抗原 See all CXCL9 ELISA试剂盒
    CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    20-6000 pg/mL
    最低检测浓度
    20 pg/mL
    应用范围
    ELISA
    原理
    Human MIG (CXCL9) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    灵敏度
    20 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our top product CXCL9 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 - 10 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    试剂准备
    1. Bring all reagents and samples to room temperature (18 - 25°C) before use. 2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) is used for dilution of serum/plasma samples, and Assay Diluent B (Item E) is used for dilution of culture supernatants and urine. 3. Assay Diluent B should be diluted 5-fold with deionized or distilled water. 4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µl Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 160 ng/ml standard. Dissolve the powder thoroughly by a gentle mix. Add 30 µl MIG standard from the vial of Item C, into a tube with 770 µl Assay Diluent A or 1x Assay Diluent B to prepare a 6000 pg/ml stock standard solution. Pipette 400 µl Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series (shown below). Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/ml). 5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer. 6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µl of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4°C for 5 days). The detection antibody concentrate should be diluted 65-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure. 7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) and pipette up and down to mix gently before use. HRP-Streptavidin concentrate should be diluted 20,000-fold with 1x Assay Diluent B before use. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 2 µl of HRP-Streptavidin concentrate into a tube with 198.0 µl 1x Assay Diluent B to prepare a 100-fold diluted HRP-Streptavidin solution (do not store the diluted solution for next day use). Mix through and then pipette 60 µl of prepared 100-fold diluted solution into a tube with 12 ml 1x Assay Diluent B to prepare a final 20,000 fold diluted HRP-Streptavidin solution.
    实验流程
    1. Bring all reagents and samples to room temperature (18 - 25°C) before use. It is recommended that all standards and samples be run at least in duplicate. 2. Add 100 µl of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4°C with gentle shaking. 3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 µl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels. 4. Add 100 µl of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking. 5. Discard the solution. Repeat the wash as in step 3. 6. Add 100 µl of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking. 7. Discard the solution. Repeat the wash as in step 3. 8. Add 100 µl of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking. 9. Add 50 µl of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    结果分析

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.

    限制
    仅限研究用
  • 储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Spaks, Jaunalksne, Spaka, Chudasama, Pirtnieks, Krievins: "Diagnostic Value of Circulating CXC Chemokines in Non-small Cell Lung Cancer." in: Anticancer research, Vol. 35, Issue 12, pp. 6979-83, (2016) (PubMed).

    Edvardsen, Bjånesøy, Hellesen, Breivik, Bakke, Husebye, Bratland: "Peripheral Blood Cells from Patients with Autoimmune Addison's Disease Poorly Respond to Interferons In Vitro, Despite Elevated Serum Levels of Interferon-Inducible Chemokines." in: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, Vol. 35, Issue 10, pp. 759-70, (2015) (PubMed).

    García, Cidoncha, Bote, Hinchado, Ortega: "Altered profile of chemokines in fibromyalgia patients." in: Annals of clinical biochemistry, Vol. 51, Issue Pt 5, pp. 576-81, (2014) (PubMed).

    Hamilton, Tower, Jones: "Identification of chemokines associated with the recruitment of decidual leukocytes in human labour: potential novel targets for preterm labour." in: PLoS ONE, Vol. 8, Issue 2, pp. e56946, (2013) (PubMed).

    De Muro, Faedda, Masala, Lepedda, Zinellu, Ciccarese, Cossu, Pala, Satta, Formato: "Kidney post-transplant monitoring of urinary glycosaminoglycans/proteoglycans and monokine induced by IFN-γ (MIG)." in: Clinical and experimental medicine, Vol. 13, Issue 1, pp. 59-65, (2013) (PubMed).

    Bratland, Hellesen, Husebye: "Induction of CXCL10 chemokine in adrenocortical cells by stimulation through toll-like receptor 3." in: Molecular and cellular endocrinology, Vol. 365, Issue 1, pp. 75-83, (2013) (PubMed).

  • 抗原 See all CXCL9 ELISA试剂盒
    CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
    别名
    MIG (CXCL9 产品)
    别名
    CMK ELISA Kit, Humig ELISA Kit, MIG ELISA Kit, SCYB9 ELISA Kit, crg-10 ELISA Kit, BB139920 ELISA Kit, Mig ELISA Kit, MuMIG ELISA Kit, Scyb9 ELISA Kit, C-X-C motif chemokine ligand 9 ELISA Kit, chemokine (C-X-C motif) ligand 9 ELISA Kit, CXCL9 ELISA Kit, Cxcl9 ELISA Kit
    背景
    MIG (monokine induced by gamma-interferon) also called MIG-1. Human MIG has a length of 103 amino acids (11.7 kDa). The protein belongs to the family of chemotactic cytokines known as Chemokines (CXC-chemokines). The human MIG gene has been localized to chromosome 4q21 in the immediate vicinity (less than 16 kb) of the gene encoding IP-10. MIG is highly expressed in the papillae of psoriatic lesions and may play an important role as a mediator of T-cell recruitment and activation in psoriasis. The Human MIG ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIG in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human MIG coated on a 96-well plate. Standards and samples are pipetted into the wells and MIG present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    基因ID
    4283
    UniProt
    Q07325
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