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IL-2 ELISA 试剂盒

IL2 适用: 人 Colorimetric Sandwich ELISA 4-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
产品编号 ABIN625022
发货至: 中国
  • 抗原 See all IL-2 (IL2) ELISA试剂盒
    IL-2 (IL2) (Interleukin 2 (IL2))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    4-1500 pg/mL
    最低检测浓度
    4 pg/mL
    应用范围
    ELISA
    原理
    Human IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    样品类型
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    特异性
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    灵敏度
    < 4 pg/mL
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling IL2 ELISA Kit
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    Discover our top product IL2 ELISA Kit
  • 应用备注
    Recommended Dilution for serum and plasma samples2 fold
    样本量
    100 μL
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    试剂准备
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use.
      2. Sample dilution: If your samples need to be diluted, Assay Diluent A (Item D) should be used for dilution of serum/plasma samples. 1x Assay Diluent B (Item E) should be used for dilution of culture supernatants and urine. Suggested dilution for normal serum/plasma: 2 fold. Please note that levels of the target protein may vary between different specimens. Optimal dilution factors for each sample must be determined by the investigator.
      3. Assay Diluent B should be diluted 5-fold with deionized or distilled water.
      4. Preparation of standard: Briefly spin the vial of Item C and then add 400 µL Assay Diluent A (for serum/plasma samples) or 1x Assay Diluent B (for cell culture medium and urine) into Item C vial to prepare a 150 ng/mL standard. Dissolve the powder thoroughly by a gentle mix. Add 10 µL IL-2 standard from the vial of Item C, into a tube with 990 µL Assay Diluent A or 1x Assay Diluent B to prepare a 1500 pg/mL stock standard solution. Pipette 400 µL Assay Diluent A or 1x Assay Diluent B into each tube. Use the stock standard solution to produce a dilution series . Mix each tube thoroughly before the next transfer. Assay Diluent A or 1x Assay Diluent B serves as the zero standard (0 pg/mL). 200 µL 200 µL 200 µL 10 µL standard +990 µL 200myl 200myl 200 µL 1500 500 166.7 55.56 18.51 6.18 2.07 0 pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL pg/mL
      5. If the Wash Concentrate (20x) (Item B) contains visible crystals, warm to room temperature and mix gently until dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to yield 400 ml of 1x Wash Buffer.
      6. Briefly spin the Detection Antibody vial (Item F) before use. Add 100 µL of 1x Assay Diluent B into the vial to prepare a detection antibody concentrate. Pipette up and down to mix gently (the concentrate can be stored at 4 °C for 5 days). The detection antibody concentrate should be diluted 58-fold with 1x Assay Diluent B and used in step 4 of Part VI Assay Procedure.
      7. Briefly spin the HRP-Streptavidin concentrate vial (Item G) before use. HRP-Streptavidin concentrate should be diluted 400-fold with 1x Assay Diluent B. For example: Briefly spin the vial (Item G) and pipette up and down to mix gently . Add 37.5 µL of HRP-Streptavidin concentrate into a tube with 15 ml 1x Assay Diluent B to prepare a final 400 fold diluted HRP-Streptavidin solution (don't store the diluted solution for next day use). Mix well.
    实验流程
    1. Bring all reagents and samples to room temperature (18 - 25 °C) before use. It is recommended that all standards and samples be run at least in duplicate.
      2. Add 100 µL of each standard (see Reagent Preparation step 2) and sample into appropriate wells. Cover well and incubate for 2.5 hours at room temperature or over night at 4 °C with gentle shaking.
      3. Discard the solution and wash 4 times with 1x Wash Solution. Wash by filling each well with Wash Buffer (300 myl) using a multi-channel Pipette or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
      4. Add 100 µL of 1x prepared biotinylated antibody (Reagent Preparation step 6) to each well. Incubate for 1 hour at room temperature with gentle shaking.
      5. Discard the solution. Repeat the wash as in step
      6. Add 100 µL of prepared Streptavidin solution (see Reagent Preparation step 7) to each well. Incubate for 45 minutes at room temperature with gentle shaking.
      7. Discard the solution. Repeat the wash as in step
      8. Add 100 µL of TMB One-Step Substrate Reagent (Item H) to each well. Incubate for 30 minutes at room temperature in the dark with gentle shaking.
      9. Add 50 µL of Stop Solution (Item I) to each well. Read at 450 nm immediately.
    结果分析

    Calculate the mean absorbance for each set of duplicate standards, controls and samples, and subtract the average zero standard optical density. Plot the standard curve on log-log graph paper or using Sigma plot software, with standard concentration on the x-axis and absorbance on the y-axis. Draw the best-fit straight line through the standard points.
    Typical Data: These standard curves are for demonstration only. A standard curve must be run with each assay. Assay Diluent A Human IL-2 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10 Assay Diluent B Human IL-2 concentration (pg/mL) 1 10 100 1000 10000 O D =4 50 n m 0.01 0.1 1 10
    Sensitivity: The minimum detectable dose of IL-2 is typically less than 4 pg/mL.
    Recovery: Recovery was determined by spiking various levels of human IL-2 into human serum, plasma and cell culture media. Mean recoveries are as follows: Sample Type Average % Recovery Range ( %) Serum 94.67 82-102 Plasma 95.52 83-103 Cell culture media 96.27 84-103
    Linearity: Sample Type Serum Plasma Cell culture media 1:2 Average % of Expected 96 95 93 Range ( %) 85-103 82-102 84-103 1:4 Average % of Expected 97 96 95 Range ( %) 83-102 84-104 85-104
    Reproducibility: Intra-Assay: CV<10 % Inter-Assay: CV<12 %

    实验精密度
    Intra-Assay: CV< 10 % Inter-Assay: CV< 12 %
    限制
    仅限研究用
  • 注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -20 °C
    储存方法
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    有效期
    6 months
  • Al-Hakeim, Al-Rammahi, Al-Dujaili: "IL-6, IL-18, sIL-2R, and TNF? proinflammatory markers in depression and schizophrenia patients who are free of overt inflammation." in: Journal of affective disorders, Vol. 182, pp. 106-14, (2015) (PubMed).

    Cervia, Catalani, Belardinelli, Perrotta, Picchietti, Alimenti, Casini, Fausto, Vallesi: "The protein pheromone Er-1 of the ciliate Euplotes raikovi stimulates human T-cell activity: involvement of interleukin-2 system." in: Experimental cell research, Vol. 319, Issue 3, pp. 56-67, (2013) (PubMed).

    Zha, Chen, Yang, Shi, Li, Wu, Lu, Li: "Upregulated TCR? enhances interleukin-2 production in T-cells from patients with CML." in: DNA and cell biology, Vol. 31, Issue 11, pp. 1628-35, (2012) (PubMed).

    Hatlapatka, Moretti, Lavrentieva, Hass, Marquardt, Jacobs, Kasper: "Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions." in: Tissue engineering. Part C, Methods, Vol. 17, Issue 4, pp. 485-93, (2011) (PubMed).

    Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, (2008) (PubMed).

    Schoof, Terashima, Batter, Douville, Richie, Eberlein: "Survival characteristics of metastatic renal cell carcinoma patients treated with lymphokine-activated killer cells plus interleukin-2." in: Urology, Vol. 41, Issue 6, pp. 534-9, (1993) (PubMed).

    Reichelt: "[The effect of ethionine on the enchondral ossification in rats]." in: Frankfurter Zeitschrift für Pathologie, Vol. 76, Issue 1, pp. 41-51 (PubMed).

  • 抗原 See all IL-2 (IL2) ELISA试剂盒
    IL-2 (IL2) (Interleukin 2 (IL2))
    别名
    IL-2 (IL2 产品)
    别名
    IL2 ELISA Kit, IL-2 ELISA Kit, il2 ELISA Kit, TCGF ELISA Kit, lymphokine ELISA Kit, Il-2 ELISA Kit, POIL2 ELISA Kit, interleukin 2 ELISA Kit, interleukin-15 ELISA Kit, IL2 ELISA Kit, il2 ELISA Kit, LOC100303688 ELISA Kit, Il2 ELISA Kit
    背景
    IL-2 is a protein of 133 amino acids (15.4 kDa). It is produced mainly by T-cells expressing the surface antigen CD4 following cell activation by mitogens or allogens under physiological conditions. IL-2 displays significant anti-tumor activity for a variety of tumor cell types since it supports the proliferation and clonal expansion of T-cells that specifically attack certain tumor types. The Human IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-2 in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for human IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Reproducibility: Intra-Assay: CV<10% Inter-Assay: CV<12%.
    基因ID
    3558
    UniProt
    P60568
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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