PCSK9 ELISA 试剂盒
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- 抗原 See all PCSK9 ELISA试剂盒
- PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.62 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.62 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9). No significant cross-reactivity or interference between Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) and analogues was observed.
- 灵敏度
- 6.4 pg/mL
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Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA Kit
PCSK9 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Competition ELISA 0.45 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 大鼠 Colorimetric Competition ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 豚鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 兔 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9) ELISA KitPCSK9 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PCSK9 ELISA试剂盒
- PCSK9 (Proprotein Convertase Subtilisin/kexin Type 9 (PCSK9))
- 别名
- Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9 产品)
- 别名
- FH3 ELISA Kit, HCHOLA3 ELISA Kit, LDLCQ1 ELISA Kit, NARC-1 ELISA Kit, NARC1 ELISA Kit, PC9 ELISA Kit, AI415265 ELISA Kit, AI747682 ELISA Kit, Narc1 ELISA Kit, proprotein convertase subtilisin/kexin type 9 ELISA Kit, proprotein convertase subtilisin/kexin type 9 L homeolog ELISA Kit, PCSK9 ELISA Kit, pcsk9.L ELISA Kit, pcsk9 ELISA Kit, Pcsk9 ELISA Kit
- 背景
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Gene Name: Proprotein Convertase Subtilisin/Kexin Type 9
Gene Aliases: FH3, HCHOLA3, NARC1, Hypercholesterolemia,Autosomal Dominant 3, Neural apoptosis-regulated convertase 1, Proprotein convertase 9, Subtilisin/kexin-like protease PC9
- 基因ID
- 100102
- UniProt
- Q80W65
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