PD-L1 ELISA 试剂盒
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). No significant cross-reactivity or interference between Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) and analogues was observed.
- 灵敏度
- 0.055 ng/mL
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CD274 (PD-L1) ELISA Kit
PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
CD274 (PD-L1) ELISA KitWide Range PD-L1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD274 (PD-L1) ELISA KitPD-L1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
PD-L1 Inhibitor Screening ELISA KitELISA, ScA 适用: 人 Colorimetric
CD274 (PD-L1) ELISA KitPD-L1 适用: 小鼠 AA 19-239 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD274 (PD-L1) ELISA KitPD-L1 适用: 人 AA 19-238 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all PD-L1 ELISA试剂盒
- PD-L1 (CD274 (PD-L1))
- 别名
- Programmed Cell Death Protein 1 Ligand 1 (PD-L1 产品)
- 别名
- B7-H ELISA Kit, B7H1 ELISA Kit, PD-L1 ELISA Kit, PDCD1L1 ELISA Kit, PDCD1LG1 ELISA Kit, PDL1 ELISA Kit, A530045L16Rik ELISA Kit, B7h1 ELISA Kit, Pdcd1l1 ELISA Kit, Pdcd1lg1 ELISA Kit, Pdl1 ELISA Kit, RGD1566211 ELISA Kit, CD274 molecule ELISA Kit, CD274 antigen ELISA Kit, programmed cell death 1 ligand 2 ELISA Kit, CD274 ELISA Kit, Cd274 ELISA Kit, PDCD1LG2 ELISA Kit
- 背景
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Gene Name: Programmed Cell Death Protein 1 Ligand 1
Gene Aliases: CD274, PDL1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1
- 基因ID
- 60533
- UniProt
- Q9EP73
- 途径
- Cancer Immune Checkpoints
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