SULT1A1 ELISA 试剂盒
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- 抗原 See all SULT1A1 ELISA试剂盒
- SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Phenol Sulfotransferase (PST). No significant cross-reactivity or interference between Phenol Sulfotransferase (PST) and analogues was observed.
- 灵敏度
- 0.059 ng/mL
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Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1) ELISA Kit
SULT1A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1) ELISA KitSULT1A1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1) ELISA KitSULT1A1 适用: 人 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1) ELISA KitSULT1A1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1) ELISA KitSULT1A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phenol Sulfotransferase (PST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phenol Sulfotransferase (PST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phenol Sulfotransferase (PST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phenol Sulfotransferase (PST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phenol Sulfotransferase (PST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all SULT1A1 ELISA试剂盒
- SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))
- 别名
- Phenol Sulfotransferase (SULT1A1 产品)
- 别名
- HAST1/HAST2 ELISA Kit, P-PST ELISA Kit, PST ELISA Kit, ST1A1 ELISA Kit, ST1A3 ELISA Kit, STP ELISA Kit, STP1 ELISA Kit, TSPST1 ELISA Kit, AI266890 ELISA Kit, Stp ELISA Kit, Stp1 ELISA Kit, ASTIV ELISA Kit, Mx-ST ELISA Kit, PST-1 ELISA Kit, St1a1 ELISA Kit, Stm ELISA Kit, Sult1a3 ELISA Kit, pst ELISA Kit, st1a3 ELISA Kit, stp ELISA Kit, stp1 ELISA Kit, tspst1 ELISA Kit, cSULT1A1 ELISA Kit, SULT1A2 ELISA Kit, SULT1A1 ELISA Kit, SIAT8-D ELISA Kit, ST8SiaIV ELISA Kit, Siat8d ELISA Kit, PST1 ELISA Kit, SIAT8D ELISA Kit, ST8SIA-IV ELISA Kit, sulfotransferase family 1A member 1 ELISA Kit, sulfotransferase family 1A, phenol-preferring, member 1 ELISA Kit, sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 ELISA Kit, sulfotransferase family 1A member 1 S homeolog ELISA Kit, sulfotransferase 1A1 ELISA Kit, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 ELISA Kit, SULT1A1 ELISA Kit, Sult1a1 ELISA Kit, sult1a1 ELISA Kit, sult1a1.S ELISA Kit, LOC704658 ELISA Kit, LOC709318 ELISA Kit, St8sia4 ELISA Kit, ST8SIA4 ELISA Kit, LOC100064221 ELISA Kit
- 背景
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Gene Name: Phenol Sulfotransferase
Gene Aliases: SULT1A1, HAST1/HAST2, ST1A3, STP1, TSPST1, Sulfotransferase 1A1, Aryl sulfotransferase 1, Phenol sulfotransferase 1, Thermostable phenol sulfotransferase
- 基因ID
- 83783
- UniProt
- P17988
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