Neurotensin ELISA 试剂盒
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- 抗原 See all Neurotensin (NTS) ELISA试剂盒
- Neurotensin (NTS)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 37.0 pg/mL - 3000 pg/mL
- 最低检测浓度
- 37.0 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Neurotensin (NT). No significant cross-reactivity or interference between Neurotensin (NT) and analogues was observed.
- 灵敏度
- 14.1 pg/mL
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Neurotensin (NTS) ELISA Kit
NTS 适用: 人 Colorimetric Competition ELISA 37.0 pg/mL - 3000 pg/mL Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Competition ELISA 37.0 pg/mL - 3000 pg/mL Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Competition ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 人 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 小鼠 Colorimetric Competition ELISA 1.56 pg/mL - 100 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitNTS 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Neurotensin (NTS) ELISA KitHigh Sensitivity NTS 适用: 人 Colorimetric Competition ELISA 3.7 pg/mL - 300 pg/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurotensin (NT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurotensin (NT) and unlabeled Neurotensin (NT) (Standards or samples) with the pre-coated antibody specific to Neurotensin (NT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurotensin (NT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurotensin (NT) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurotensin (NT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurotensin (NT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Neurotensin (NTS) ELISA试剂盒
- Neurotensin (NTS)
- 别名
- Neurotensin (NTS 产品)
- 别名
- NTS ELISA Kit, NMN-125 ELISA Kit, NN ELISA Kit, NT ELISA Kit, NT/N ELISA Kit, NTS1 ELISA Kit, 5033428E16Rik ELISA Kit, neurotensin ELISA Kit, NTS ELISA Kit, Nts ELISA Kit
- 背景
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Gene Name: Neurotensin
Gene Aliases: NTS, NTS1, NmN-125, NN, Tail peptide, Large neuromedin N
- 基因ID
- 4922
- UniProt
- P30990
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