电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

KARS ELISA 试剂盒

KARS 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5656570
发货至: 中国
  • 抗原 See all KARS ELISA试剂盒
    KARS (Lysyl-tRNA Synthetase (KARS))
    适用
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Lysyl tRNA Synthetase (KARS). No significant cross-reactivity or interference between Lysyl tRNA Synthetase (KARS) and analogues was observed.
    灵敏度
    0.117 ng/mL
    Featured
    Discover our best selling KARS ELISA Kit
    Top Product
    Discover our top product KARS ELISA Kit
  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysyl tRNA Synthetase (KARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lysyl tRNA Synthetase (KARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysyl tRNA Synthetase (KARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysyl tRNA Synthetase (KARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lysyl tRNA Synthetase (KARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all KARS ELISA试剂盒
    KARS (Lysyl-tRNA Synthetase (KARS))
    别名
    Lysyl tRNA Synthetase (KARS 产品)
    别名
    CG12141 ELISA Kit, Dmel\\CG12141 ELISA Kit, KRS ELISA Kit, LysRS ELISA Kit, cb530 ELISA Kit, wu:fa16h02 ELISA Kit, zgc:92483 ELISA Kit, kars ELISA Kit, MGC53345 ELISA Kit, kars2 ELISA Kit, krs ELISA Kit, krs-1 ELISA Kit, KARS ELISA Kit, Tb06.28P18.190 ELISA Kit, CMTRIB ELISA Kit, DFNB89 ELISA Kit, KARS1 ELISA Kit, KARS2 ELISA Kit, AA589550 ELISA Kit, AL024334 ELISA Kit, AL033315 ELISA Kit, AL033367 ELISA Kit, D8Ertd698e ELISA Kit, D8Wsu108e ELISA Kit, mKIAA0070 ELISA Kit, Lysyl-tRNA synthetase ELISA Kit, lysyl-tRNA synthetase ELISA Kit, lysyl-tRNA synthetase S homeolog ELISA Kit, lysyl-tRNA synthetase L homeolog ELISA Kit, LysRS ELISA Kit, kars ELISA Kit, kars.S ELISA Kit, kars.L ELISA Kit, Bm1_16500 ELISA Kit, lysS ELISA Kit, Taci_1118 ELISA Kit, Tpau_1737 ELISA Kit, Arch_0261 ELISA Kit, Trad_2960 ELISA Kit, Spirs_3699 ELISA Kit, KARS ELISA Kit, LOC100280510 ELISA Kit, Tb927.6.1510 ELISA Kit, Kars ELISA Kit
    背景

    Gene Name: Lysyl tRNA Synthetase

    Gene Aliases: KARS2, LysRS, Lysine tRNA Ligase

    基因ID
    3735
    UniProt
    Q15046
You are here:
客服