Hemoglobin Subunit beta ELISA 试剂盒
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- 抗原 See all Hemoglobin Subunit beta (HBB) ELISA试剂盒
- Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Competition ELISA
- 检测范围
- 6.17 μg/mL - 500 μg/mL
- 最低检测浓度
- 6.17 μg/mL
- 应用范围
- ELISA
- 样品类型
- Erythrocyte Lysates, Plasma, Serum
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Hemoglobin Beta (HBb). No significant cross-reactivity or interference between Hemoglobin Beta (HBb) and analogues was observed.
- 灵敏度
- 2.88 μg/mL
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Hemoglobin beta (HBB) ELISA Kit
HBB 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 6.17 μg/mL - 500 μg/mL Erythrocyte Lysates, Plasma, Serum
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 1.56 μg/mL - 100 μg/mL Cell Lysate, Plasma, Serum
Hemoglobin beta (HBB) ELISA KitHBB 适用: 猴 Colorimetric Sandwich ELISA 0.12-500 ng/mL Red Blood Cell Lysate
Hemoglobin beta (HBB) ELISA KitHBB 适用: 人 Colorimetric Competition ELISA 0.12-500 ng/mL Plasma, Red Blood Cell Lysate, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 2 h
- 板类型
- Pre-coated
- 实验流程
- This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hemoglobin Beta (HBb) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hemoglobin Beta (HBb) and unlabeled Hemoglobin Beta (HBb) (Standards or samples) with the pre-coated antibody specific to Hemoglobin Beta (HBb). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Hemoglobin Beta (HBb) in the sample.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin Beta (HBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Hemoglobin Subunit beta (HBB) ELISA试剂盒
- Hemoglobin Subunit beta (HBB) (Hemoglobin beta (HBB))
- 别名
- Hemoglobin Beta (HBB 产品)
- 别名
- HBB ELISA Kit, HBB1 ELISA Kit, HBBA ELISA Kit, HBBB ELISA Kit, CD113t-C ELISA Kit, beta-globin ELISA Kit, hbb ELISA Kit, hemoglobin, beta ELISA Kit, hemoglobin subunit beta ELISA Kit, hemoglobin subunit beta-like ELISA Kit, beta globin ELISA Kit, hemoglobin beta chain complex ELISA Kit, hemoglobin subunit beta-1 ELISA Kit, HBB ELISA Kit, HBB2 ELISA Kit, LOC100720944 ELISA Kit, LOC480784 ELISA Kit, Hbb ELISA Kit, LOC100136576 ELISA Kit, LOC101098159 ELISA Kit, LOC100976465 ELISA Kit, LOC100410611 ELISA Kit, LOC105468624 ELISA Kit
- 物质类
- Viral Protein
- 背景
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Gene Name: Hemoglobin Beta
Gene Aliases: HB-B, HBD, CD113t-C, Beta Globin, Spinorphin
- 基因ID
- 3043
- UniProt
- P68871
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