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GOLM1 ELISA 试剂盒

GOLM1 适用: 人 Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5654787
发货至: 中国
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    GOLM1 (Golgi Membrane Protein 1 (GOLM1))
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.625 ng/mL - 40 ng/mL
    最低检测浓度
    0.625 ng/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    特异性
    This assay has high sensitivity and excellent specificity for detection of Golgi Protein 73 (GP73). No significant cross-reactivity or interference between Golgi Protein 73 (GP73) and analogues was observed.
    灵敏度
    0.229 ng/mL
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  • 说明

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    实验时间
    3 h
    板类型
    Pre-coated
    实验流程
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Golgi Protein 73 (GP73). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Golgi Protein 73 (GP73). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Golgi Protein 73 (GP73), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Golgi Protein 73 (GP73) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    实验精密度
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Golgi Protein 73 (GP73) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    限制
    仅限研究用
  • 注意事项
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    储存条件
    4 °C,-20 °C
    储存方法
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    有效期
    4-8 months
  • 抗原 See all GOLM1 ELISA试剂盒
    GOLM1 (Golgi Membrane Protein 1 (GOLM1))
    别名
    Golgi Protein 73 (GOLM1 产品)
    别名
    C9orf155 ELISA Kit, GOLPH2 ELISA Kit, GP73 ELISA Kit, PSEC0257 ELISA Kit, bA379P1.3 ELISA Kit, 2310001L02Rik ELISA Kit, AW125446 ELISA Kit, D030064E01Rik ELISA Kit, Golph2 ELISA Kit, GOLM1 ELISA Kit, golph2 ELISA Kit, gp73 ELISA Kit, golgi membrane protein 1 ELISA Kit, SPATA31 subfamily D, member 3 ELISA Kit, Golgi membrane protein 1 ELISA Kit, golgi membrane protein 1 L homeolog ELISA Kit, GOLM1 ELISA Kit, Golm1 ELISA Kit, Spata31d3 ELISA Kit, golm1 ELISA Kit, LOC100400549 ELISA Kit, golm1.L ELISA Kit
    背景

    Gene Name: Golgi Protein 73

    Gene Aliases: GOLM1, GOLPH2, C9orf155, Golgi Membrane Protein 1, Golgi Phosphoprotein 2

    基因ID
    51280
    UniProt
    Q8NBJ4
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