Coagulation Factor IX ELISA 试剂盒
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- 抗原 See all Coagulation Factor IX (F9) ELISA试剂盒
- Coagulation Factor IX (F9)
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.12 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.12 pg/mL
- 应用范围
- ELISA
- 样品类型
- Plasma
- Analytical Method
- Quantitative
- 特异性
- This assay has high sensitivity and excellent specificity for detection of Coagulation Factor IX (F9). No significant cross-reactivity or interference between Coagulation Factor IX (F9) and analogues was observed.
- 灵敏度
- 32 pg/mL
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Coagulation Factor IX (F9) ELISA Kit
F9 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor IX (F9) ELISA KitF9 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma
Coagulation Factor IX (F9) ELISA KitF9 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor IX (F9) ELISA KitF9 适用: 人 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor IX (F9) ELISA KitF9 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Coagulation Factor IX (F9) ELISA KitF9 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor IX (F9) ELISA KitF9 适用: 大鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Coagulation Factor IX (F9) ELISA KitF9 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma
Coagulation Factor IX (F9) ELISA KitF9 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma
Coagulation Factor IX (F9) ELISA KitF9 适用: 猴 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
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- 说明
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- 实验时间
- 3 h
- 板类型
- Pre-coated
- 实验流程
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor IX (F9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor IX (F9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor IX (F9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor IX (F9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- 实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor IX (F9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - 限制
- 仅限研究用
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- 注意事项
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- 有效期
- 4-8 months
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- 抗原 See all Coagulation Factor IX (F9) ELISA试剂盒
- Coagulation Factor IX (F9)
- 别名
- Coagulation Factor IX (F9 产品)
- 别名
- AA407498 ELISA Kit, AI506714 ELISA Kit, AK-3 ELISA Kit, Ak3l ELISA Kit, Ak3l1 ELISA Kit, Akl3l ELISA Kit, FIX ELISA Kit, HEMB ELISA Kit, P19 ELISA Kit, PTC ELISA Kit, THPH8 ELISA Kit, AW111646 ELISA Kit, Cf-9 ELISA Kit, Cf9 ELISA Kit, f9 ELISA Kit, zgc:109775 ELISA Kit, fix ELISA Kit, ptc ELISA Kit, hemb ELISA Kit, FIXA ELISA Kit, zgc:136807 ELISA Kit, adenylate kinase 3 ELISA Kit, coagulation factor IX ELISA Kit, coagulation factor IXa ELISA Kit, coagulation factor 9 ELISA Kit, coagulation factor 9 S homeolog ELISA Kit, Coagulation factor IX ELISA Kit, coagulation factor IXb ELISA Kit, Ak3 ELISA Kit, F9 ELISA Kit, f9a ELISA Kit, f9 ELISA Kit, CpipJ_CPIJ003776 ELISA Kit, CpipJ_CPIJ009129 ELISA Kit, CpipJ_CPIJ013063 ELISA Kit, CpipJ_CPIJ013624 ELISA Kit, Tsp_01441 ELISA Kit, f9.S ELISA Kit, fa9 ELISA Kit, f9b ELISA Kit
- 背景
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Gene Name: Coagulation Factor IX
Gene Aliases: HEMB, FIX, GLA Domain, PTC, Anti Hemophilic Factor B, Christmas Factor, Plasma Thromboplastic Component, Christmas Disease, Hemophilia B
- 基因ID
- 2158
- UniProt
- P00740
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